| 抗CDV、CAV卵黄抗体的制备 |
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| 引用本文： | 王玉燕,朱瑞良.抗CDV、CAV卵黄抗体的制备[J].中国预防兽医学报,2003,25(3):215-218. |
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| 作者姓名： | 王玉燕 朱瑞良 |
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| 作者单位： | 山东农业大学,动物科技学院,山东,泰安,271018 |
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| 摘 要： | 本研究用CDV、CAV分别免疫产蛋鸡，收集鸡蛋，用海藻酸钠—硫酸铵法提取卵黄抗体(IgY)，并对提取的IgY采用了SDS-PAGE和低压层析检测纯度，结果表明用该法提取的IgY纯度较高。用间接BUSA测定提取的IgY活性，结果证明提取的抗CAV IgY的活性较高，而抗CDV IgY的活性损失较大。
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| 关 键 词： | 犬瘟热病毒 犬腺病毒 海藻酸钠 卵黄抗体 制备 检测 |
| 文章编号： | 1008-0589(2003)03-0215-04 |
| 修稿时间： | 2002年8月12日 |
Extraction of yolk immunoglobulin against CDV,CAV |
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| WANG Yu_yan,ZHU Rui_liang.Extraction of yolk immunoglobulin against CDV,CAV[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(3):215-218. |
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| Authors: | WANG Yu_yan ZHU Rui_liang |
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| Institution: | WANG Yu_yan,ZHU Rui_liang * |
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| Abstract: | Two groups of hens were immunized by CDV,CAV respectively,and eggs were collected.IgY was salting out by the method of sodium alginic acid combined ammoumium sulface.The SDS_PAGE and the system of low pressture chromatography were used to detected the purity of IgY,and the results suggested that there are two protein components in the extracted IgY at least.The indirect ELISA was used to assay the activity of IgY against CDV,CAV,and the results showed that the activity to IgY against CAV was higher,but the activity of IgY against CDV loss much. |
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| Keywords: | canine distemper virus canine adenovirus sodium alginic acid yolk immunoglobulin extraction detection |
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