| 黑褐新糠虾血细胞形态及其与日本新糠虾对溶藻弧菌敏感性的比较 |
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| 引用本文： | 赵柳兰,杨筱珍,范朋,杨丽丽,张金彪,梁攀,洪宇航,成永旭.黑褐新糠虾血细胞形态及其与日本新糠虾对溶藻弧菌敏感性的比较[J].水产学报,2011,35(11):1711-1719. |
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| 作者姓名： | 赵柳兰 杨筱珍 范朋 杨丽丽 张金彪 梁攀 洪宇航 成永旭 |
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| 作者单位： | 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海市高校水产养殖学E-研究院,上海201306 |
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| 基金项目： | 国家自然科学基金项目(30700609);上海市重点学科建设项目(Y1101);上海高校创新团队(第二期)项目 |
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| 摘 要： | 实验采用Giemsa与Wright' s两种染色方法对黑褐新糠虾血细胞进行染色,根据血细胞大小,所含颗粒与否,颗粒多少和核质比将其分型,并对各型血细胞所占比例进行统计.结果表明,通过比较Giemsa染色与Wright's染色两种方法的染色时间和效果,Wright's染色更加适合糠虾血细胞.通过染色,将黑褐新糠虾血细胞分为3种类型,它们分别为透明细胞、半颗粒细胞和颗粒细胞.它们的胞体大小依次增大,核质比依次降低.对此3种类型血细胞比例分别统计后发现,颗粒细胞所占比例最多约( 48.38％±0.05％),其次是半颗粒细胞约为(35.22％±0.04％),最少的为透明细胞约为(16.40％±0.01％).对不同浓度溶藻弧菌的感染试验发现,日本新糠虾在较短的时间内出现死亡,即48h,而黑褐新糠虾72 h出现死亡,各浓度组中日本新糠虾死亡率均高于黑褐新糠虾.黑褐新糠虾相对日本新糠虾来说,对溶藻弧菌具有较低的敏感性,可能与其血细胞中颗粒和半颗粒比例较高有关.
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| 关 键 词： | 黑褐新糠虾 日本新糠虾 溶藻弧菌 血细胞 Giemsa染色 Wright's染色 |
| 收稿时间： | 2011/2/22 0:00:00 |
| 修稿时间： | 2011/8/19 0:00:00 |
The study on the haemocyte morphology of the adult Neomysis awatschensis and the different susceptibility to Vibrio alginolyticus compared with Neomysis japonica |
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| ZHAO Liu lan,YANG Xiao zhen,FAN Peng,YANG Li li,ZHANG Jin biao,LIANG Pan,HONG Yu hang and CHENG Yong xu.The study on the haemocyte morphology of the adult Neomysis awatschensis and the different susceptibility to Vibrio alginolyticus compared with Neomysis japonica[J].Journal of Fisheries of China,2011,35(11):1711-1719. |
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| Authors: | ZHAO Liu lan YANG Xiao zhen FAN Peng YANG Li li ZHANG Jin biao LIANG Pan HONG Yu hang and CHENG Yong xu |
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| Institution: | ZHAO Liu-lan,YANG Xiao-zhen,FAN Peng,YANG Li-li,ZHANG Jin-biao,LIANG Pan,HONG Yu-hang,CHENG Yong-xu(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources and Aquaculture Division,Ministry of Education,E-Institute of Shanghai Universities,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | In consideration of the differences between Neomysis awatschensis and Neomysis japonica in the growth,reproduction,susceptibility and other aspects,the susceptibility of two Neomysis to Vibrio alginolyticus was studied,which could help to explore to the root of the phenomenon in immunology.Two staining methods of Giemsa and wright's were used in this experiment of the haemocytes of the adult N.awatschensis,and haemocytes of the mysis were classified base on the large/small size,the presence/absence of cytop... |
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| Keywords: | Neomysis awatschensis Neomysis japonica Vibrio alginolyticus haemocytes Giemsa staining Wright's staining |
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