HPLC separation and subsequent detection of aromatic heptaene polyenes in peat after treatment with Streptomyces griseoviridis |
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| Authors: | Olavi J Raatikainen Tuulikki H Pivinen Risto T Tahvonen |
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| Institution: | Olavi J. Raatikainen,Tuulikki H. Päivinen,Risto T. Tahvonen |
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| Abstract: | Aromatic heptaene polyenes (AHPs), the largest group of antifungal polyene antibiotics, were detected in steam-sterilized Sphagnum peat after treatment with a new biofungicide containing fermenter-cultivated Streptomyces griseoviridis Anderson K61, a bacterium capable of producing AHPs in vitro. Three concentrations (0.05 g litre?1, 0-2 g litre?1 and 2.0 g litre?1) of the biofungicide were used and compared to untreated controls. The substrates were incubated for seven weeks under greenhouse conditions, with and without cucumber seedlings, and samples were analysed for heptaene polyenes by HPLC using a photodiode array detector. Changes in AHP concentrations indicated that the fermenter-cultivated mycelium was a partial source of extractable heptaenes in peat. The identification of p-aminoacetophenone (PAAP) by HPLC-MS in peat hydrolysates verified the presence of AHPs. |
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