| 进境澳大利亚大麦夹杂油菜茎基溃疡病菌的检疫鉴定 |
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| 引用本文： | 张慧丽,李雪莲,陈先锋,吕 燕,段维军.进境澳大利亚大麦夹杂油菜茎基溃疡病菌的检疫鉴定[J].植物保护,2021,47(4):52-58. |
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| 作者姓名： | 张慧丽 李雪莲 陈先锋 吕 燕 段维军 |
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| 作者单位： | 1. 宁波检验检疫科学技术研究院, 宁波 315012; 2. 宁波海关, 宁波 315012 |
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| 基金项目： | 国家重点研发计划(2016YFF0203201); 浙江省公益技术研究项目(LGF20C140001); 海关总署科研项目(2020HK162); 中国检验检疫科学研究院基本科研业务费(2020JK048) |
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| 摘 要： | 采用常规平板分离法, 从进境澳大利亚大麦中夹杂的油菜籽上获得1株疑似油菜茎基溃疡病菌的菌株01829?通过致病性测定?形态学观察?特异性引物扩增?ITS序列比对分析, 对01829进行了种类鉴定?结果表明:菌株01829在PDA培养基上生长较慢, 菌落边缘不整齐, 产生大量分生孢子器和分生孢子; 采用特异性引物对LMR1-D和Lmb分别进行PCR检测, 结果均有预期扩增片段产生; 基于ITS序列构建的系统发育树中, 菌株01829和GenBank中其他油菜茎基溃疡病菌相关序列聚在同一分支; 菌株01829接种油菜子叶和茎基部, 在子叶和茎基部接种部位分别引起叶斑和凹陷溃疡斑?根据上述试验结果, 将菌株01829鉴定为油菜茎基溃疡病菌, 这是我国口岸首次从进境澳大利亚大麦中截获油菜茎基溃疡病菌?
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| 关 键 词： | 油菜茎基溃疡病菌 形态特征 系统发育分析 致病性测定 鉴定 |
| 收稿时间： | 2020/3/5 0:00:00 |
| 修稿时间： | 2020/5/15 0:00:00 |
Quarantine identification of Leptosphaeria maculans on Hordeum vulgare imported from Australia |
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| Institution: | 1. Ningbo Academy of Inspection and Quarantine, Ningbo 315012, China; 2. Ningbo Customs, Ningbo 315012, China |
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| Abstract: | The fungal strain 01829 was isolated from the oilseed rape mixed in Hordeum vulgare imported from Australia by using traditional tissue separation method. Morphological observation, specific PCR detection, ITS sequence analysis and pathogenicity test were carried out to identify the strain 01829. The morphological results showed that the isolate 01829 grew slowly with irregular colony edges on PDA and produced abundant pycnidia and conidia. In specific PCR tests, the primer pairs LMR1-D and Lmb amplified a positive fragment from the DNA of isolate 01829. Its ITS sequence was sequenced. Analysis of the sequence with the related sequences retrieved from GenBank showed that the strain 01829 clustered into Leptosphaeria maculans clade. The pathogenicity test showed that the strain 01829 could cause leaf spot of cotyledons and canker lesion of stem. Based on these results, the isolate 01829 was identified as L.maculans. To our knowledge, this is the first interception report of L.maculans mixed into H.vulgare imported from Australia to China. |
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| Keywords: | Leptosphaeria maculans morphological characteristics phylogenetic analysis pathogenicity test identification |
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