Validation of molecular markers linked to fertility restorer gene(s) for WA-CMS lines of rice |
| |
| Authors: | N K Sheeba B C Viraktamath S Sivaramakrishnan M G Gangashetti Pawan Khera R M Sundaram |
| |
| Institution: | (1) Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad, Andhra Pradesh, India;(2) Directorate of Rice Research, Hyderabad, Andhra Pradesh, India;(3) Barwale Foundation (formerly known as MRF), Banjara Hills, Hyderabad, Andhra Pradesh, India |
| |
| Abstract: | The present study was carried out with the objective to validate the molecular markers, which have been previously reported
to be linked to fertility restorer (Rf) gene(s) for WA-CMS lines of rice. Two mapping populations involving fertility restorer lines for WA-cytoplasm, viz., (i)
an F2 population derived from the cross IR58025A/KMR3R consisting of 347 plants and (ii) a BC1F1 population derived from the cross IR62829A/IR10198R//IR62829A consisting of 130 plants were analyzed. Nine SSR and three
CAPS markers reported to be linked to Rf genes along with two previously unreported SSR markers were analyzed in the mapping populations. In both the populations
studied, the trait of fertility restoration was observed to be under digenic control. Eight SSR markers (RM6100, RM228, RM171,
RM216, RM474, RM311, MRG4456 and pRf1&2) showed polymorphism between the parents of the F2 population, while the SSR markers RM6100 and RM474 showed polymorphism between the parents of both the F2 and BC1F1 populations. Only one CAPS marker, RG146FL/RL was polymorphic between the parents of the BC1F1 population. RM6100 was observed to be closely segregating with fertility restoration in both the mapping populations and
was located at a distance of ~1.2 cM. The largest phenotypic variation was accounted for the region located between RM311
and RM6100. Using the marker-trait segregation data derived from analysis of both the mapping populations, a local linkage
map of the genomic region around Rf-4, a major fertility restoration locus on Chromosome 10 was constructed, and RM6100 was observed to be very close to the gene
at a distance of 1.2 cM. The accuracy of the marker RM6100 in predicting fertility restoration was validated in 21 restorers
and 18 maintainers. RM6100 amplified the Rf-4 linked allele in a majority of the restorers with a selection accuracy of 94.87%. Through the present study, we have established
the usefulness of the marker RM6100 in marker-assisted selection for fertility restoration in segregating populations and
identification of restorers while screening rice germplasm for their fertility restoration ability. |
| |
| Keywords: | Cytoplasmic male sterility Wild abortive cytoplasm Fertility restorer genes SSRs CAPS |
| 本文献已被 SpringerLink 等数据库收录！ |
|
