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| 蜡状芽孢杆菌抗重金属性能及对镉的累积 | |
| 引用本文： | 刘红娟,党志,张慧,易筱筠,刘丛强.蜡状芽孢杆菌抗重金属性能及对镉的累积[J].农业环境科学学报,2010,29(1). |
| 作者姓名： | 刘红娟 党志 张慧 易筱筠 刘丛强 |
| 作者单位： | 1. 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广东,广州,510006;南华大学核科学技术学院,湖南,衡阳,421001 2. 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广东,广州,510006 3. 中国科学院环境地球化学研究所,环境地球化学国家重点实验室,贵州,贵阳,550002 |
| 基金项目： | 国家自然科学基金重点项目(40730741);;广东省自然科学基金研究团队项目(9351064101000001);;广东省科技计划国际合作项目(2007A050100023);;环境地球化学国家重点实验室开放基金 |
| 摘 要： | 所研究的蜡状芽孢杆菌RC可以在镉浓度为200mg·L-1的固体培养基平板上生长良好,表明菌株具有强抗镉的能力。该菌株在液体培养基中Cd2+、Cr3+、Pb2+浓度均为75mg·L-1和Mn2+浓度为100mg·L-1培养时,菌株生长正常。在重金属Cr3+、Pb2+、Mn2+存在时,采用红外光谱与原子吸收光谱分析菌株对Cd2+的积累,结果表明,培养基中其他重金属离子的加入,会影响菌株对Cd2+的积累率;当Cr3+存在时,Cr3+可以增加细胞壁上有效官能团活性,明显提高RC菌体对Cd2+的积累率,而其他重金属组合对Cd2+吸附积累能力影响不大;RC细胞壁上活性基团-OH、-NH-、-COOH、-PO34-和-M-O(O-M-O)活跃参与Cr3+、Pb2+、Mn2+和Cd2+多种重金属离子的络合作用。通过高温和十二烷基硫酸钠处理菌株进行质粒消除试验,未发现该菌株的抗镉性质与抗性质粒的存在相关。 |
| 关 键 词： | 蜡状芽孢杆菌 镉 抗性 红外光谱 |
Characterization of Heavy Metal Resistance and Cd-accumulation on a Bacillus cereus Strain |
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| LIU Hong-juan,DANG Zhi,ZHANG Hui,YI Xiao-yun,LIU Cong-qiang.Characterization of Heavy Metal Resistance and Cd-accumulation on a Bacillus cereus Strain[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2010,29(1). | |
| Authors: | LIU Hong-juan DANG Zhi ZHANG Hui YI Xiao-yun LIU Cong-qiang |
| Abstract: | The Cd-resistant Bacillus cereus RC grows well on media plates containing 200 mg. L-I cadmium. The strain grew normally in the presence of 75 mg·L~(-1) of Cd~(2+), Cr~(2+), Pb~(2+) or 100 mg·L~(-1) of Mn~(2+). The accumulation to Cd in the strain was observed by infrared spectroscopy and atomic absorption spectroscopy, in the presence of Cr~(3+), Pb~(2+) or Mn~(2+). The results indicate that the presence of the other heavy metals affected the accumulation of Cd~(2+). Cr~(2+) increased the activity of the groups and facilitated the accumulation of Cd~(2+) on the cell wall. Adsorption and accumulation varied only slightly in media containing the other metal ions. This shows that -OH, -NH, -CO, -CO-NH- and -PO_4~(3-) groups on the cell wall of RC actively participate in the complexing of Cr~(3+), Pb~(2+), Mn~(2+) and Cd~(2+) ions. A plasmid elimination experiment suggests that Cd-resistant genes were not located on its plasmid. The results of this study show that this strain is resistant to cadmium and indicate the possible usefulness of this strain for bioremediation of sites contaminated with cadmium. |
| Keywords: | Bacillus cereus cadmium resistance infrared spectroscopy |
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