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A new approach to enhance growth and nodulation of Acacia mangium through aeroponic culture
Authors:F Martin-Laurent  S-K Lee  F-Y Tham  J He  H G Diem  P Durand
Institution:(1) Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement, CIRAD, Selegie Complex #14–275, 257 Selegie Road, Singapore 188350, Singapore, SG;(2) Nanyang Technological University, National Institute of Education, Biology Division, 469 Bukit Timah Road, Singapore 259756, Singapore Fax: 469-8952, SG;(3) Centre National de la Recherche Scientifique, CNRS, Direction des Relations Internationales, 3 Rue Michel Ange, F-75794 Paris Cedex 16, France, FR
Abstract:This work was designed to determine whether a plant culture method on non-solid media could be used as an alternative for inoculation of Acacia mangium with selected strains of Bradyrhizobium spp. A. mangium seedlings were grown and inoculated with Bradyrhizobium strain Aust13c and strain Tel2 in hydroponics, aeroponics and sand. Aeroponics was found to be the best system of the three, allowing the production of tree saplings 1 m in height after only 4 months in culture. Moreover, compared to plants grown in liquid or sand media, aeroponically grown saplings inoculated with Bradyrhizobium spp. developed a very high number of small nodules distributed all along the root system, resulting in an increase in nitrogen and chlorophyll content in plant tissues. We propose aeroponics as an alternative method to classical soil inoculation procedures for the production of hypernodulated legume tree saplings. Received: 18 July 1996
Keywords:Aeroponic culture  N2-fixation  Acacia mangium  Bradyrhizobium spp    Hypernodulation  Tree saplings  Imperata cylindrica
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