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| 猪链球菌精氨酸脱亚氨酸酶序列分析及其PCR检测 | |
| 引用本文： | 张东,王楷宬,汤明,黄保续,范伟兴.猪链球菌精氨酸脱亚氨酸酶序列分析及其PCR检测[J].中国动物检疫,2007,24(8):27-29. |
| 作者姓名： | 张东 王楷宬 汤明 黄保续 范伟兴 |
| 作者单位： | 1. 西南大学动物科技学院,重庆,4007152 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 重庆市动物卫生监督总站,重庆,401147 |
| 摘 要： | 对9株猪链球菌2型重庆分离株的精氨酸脱亚氨酸酶基因进行克隆测序,结果表明该基因长度为1231bp,与Genbank发表的该基因序列相比,核苷酸同源性高于99%,推导的氨基酸同源性高于96%。根据精氨酸脱亚氨酸酶基因的测序结果建立扩增片段长度为237bp的PCR检测方法,35株猪链球菌致病株中,30株精氨酸脱亚氨酸酶基因的PCR检测阳性,有5株精氨酸脱亚氨酸酶基因的PCR检测阴性;14株正常猪扁桃体分离株中,11株为精氨酸脱亚氨酸酶基因的PCR检测阳性,3株为精氨酸脱亚氨酸酶基因的PCR检测阴性;猪链球菌1型、7型、9型、13型、1/2型各一株,均能扩增出精氨酸脱亚氨酸酶基因的片段。 |
| 关 键 词： | 猪链球菌2型 精氨酸脱亚氨酸酶 序列分析 PCR检测 |
| 文章编号： | 1005-944X（2007）08-0027-03 |
The Arg-deiminase gene of Streptococcus suis sequence analysis and PCR detection |
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| Zhang Dong,Wang Kaicheng,Tang Ming,Huang Baoxu,Fan Weixing.The Arg-deiminase gene of Streptococcus suis sequence analysis and PCR detection[J].China Journal Of Animal Quarantine,2007,24(8):27-29. | |
| Authors: | Zhang Dong Wang Kaicheng Tang Ming Huang Baoxu Fan Weixing |
| Institution: | 1.Animal Science College,Southwest University,Chongqing 400715; 2.China Animal Health and Epidemiology Center,Qingdao 266032; 3.Chongqing Animal Health Supervision General Station,Chongqing 401147 |
| Abstract: | In this study, the Arg-deiminase from 9 CHONGQING Streptococcus suis serotype 2 isolates was amplified by PCR. Nucleotide sequencing and analysis results revealed that the Arg-deiminase gene is 1,231bp. The similarity of Arg-deiminase gene and amino acid from 9 CHONGQING isolates was more than 99% and 96% respectively. The Arg-deiminase gene was detected by PCR with a 237bp expected fragment, The results showed that in 35 pathogenic Streptococcus suis isolates from diseased pigs' viscera, 30 isolates were positive; 11 isolates were positive in 14 detected Streptococcus suis isolates from pigs' tonsil. SS1, SS7, SS9, SS13 and SS1/2 detected by PCR were all positive. |
| Keywords: | Streptococcus suis serotype 2 Streptococcus suis Arg-deiminase sequence analysis PCR detection |
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