| pH试差法测定蓝靛果花色苷及大孔树脂纯化的研究 |
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| 引用本文： | 刘敬华,王振宇.pH试差法测定蓝靛果花色苷及大孔树脂纯化的研究[J].中国林副特产,2012(5):28-30. |
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| 作者姓名： | 刘敬华 王振宇 |
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| 作者单位： | 1. 东北林业大学 林学院 ,哈尔滨 150040
2. 哈尔滨工业大学 食品科学与工程学院 ,哈尔滨 150090 |
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| 摘 要： | 以一个全新视角对蓝靛果花色苷进行研究:在大孔树脂的筛选方面,通过pH试差法以直接上层析柱的方式,并以所纯化花色苷最浓部分的浓度为指标进行研究,D101的纯化效果较好;在对影响层析效果的因素方面,以所纯化花色苷最浓部分的纯度为指标对上样浓度和洗脱剂浓度两个重要指标进行研究,得到在上样浓度为1.5mg/mL、50%乙醇洗脱条件下的效果较好。
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| 关 键 词： | 蓝靛果 pH试差法 花色苷 纯化 |
Determination the Anthocyanin in Lonicera edulisby pHdifferential Spetrophotometry and Purification Research in Resin |
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| Liu Jinghua,Wang Zhenyu.Determination the Anthocyanin in Lonicera edulisby pHdifferential Spetrophotometry and Purification Research in Resin[J].Forest By-product and Speciality in China,2012(5):28-30. |
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| Authors: | Liu Jinghua Wang Zhenyu |
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| Institution: | 1.Shool of Forestry,Northeast Forestry university,Harbin 150040; 2.Sool of Food Science and Engineering,Harbin Institute of Technology,Harbin 150090) |
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| Abstract: | Anthocyanin in Lonicera edulis is studied from a new perspective in this paper.In selection of resins,These anthocyanins are directly put into chromatography column and detected the concentration of the most concentration as an index by pH-differential spetrotometry,the effect of D101 is better;in chromatographic effect factors,we take the purification of the most concentration as an index to study the two key factors of the concentration of sample and eluting agent,1.5mg/mL of sample and 50% of ethanol elution is better. |
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| Keywords: | Lonicera edulis pH-differential spetrotometry Anthocyanin Purification |
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