| 油茶单芽组培生根研究 |
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| 引用本文： | 吴幼媚,王以红,蔡玲,陈博雯,陈晓明.油茶单芽组培生根研究[J].云南林业科技,2012(4):25-28. |
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| 作者姓名： | 吴幼媚 王以红 蔡玲 陈博雯 陈晓明 |
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| 作者单位： | [1]广西林业科学研究院,广西南宁530002 [2]国家林业局中南速生材繁育重点实验室,广西南宁530002 [3]广西优良用材林资源培育重点实验室,广西南宁530002 |
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| 基金项目： | 基金项目：广西自治区主席基金（09203-05）、国家林业局推广项目（[2010]TG02）. |
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| 摘 要： | 采用基本培养基、培养温度、光照强度、单芽形态和激素等因素对油茶岑软2号无性系组培苗生根影响的筛选试验。结果表明,1/2ER为油茶组织培养最适基本培养基;ABT60.5 mg/L＋IAA 2.0 mg/L＋NAA0.8 mg/L为最优的激素浓度组合,组培单芽培养40天生根率达93.7%,每个单芽长根3～4条;在室温28℃±2℃,光强2 500～3 500 Lx条件下利于油茶组培苗的生根,生根率高达97.8%。
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| 关 键 词： | 油茶 组织培养 生根 |
Study on rooting of single buds of Camellia oleifera |
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| WU You-mei,WANG Yi-hong CAI Ling,CHEN Bo-wen,CHEN Xiao-ming.Study on rooting of single buds of Camellia oleifera[J].Yunnan Forestry Science and Technology,2012(4):25-28. |
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| Authors: | WU You-mei WANG Yi-hong CAI Ling CHEN Bo-wen CHEN Xiao-ming |
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| Institution: | .( Guangxi Forestry Research Institute, Nanning Guangxi 530002, P. R. China; 2. Key Laboratory of Central South Fast-growing Timber Cultivation State Forestry Administration, Nanning Guangxi 530002, P. R. China; 3. Gnangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Nanning Guangxi 530002, P. R. China) |
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| Abstract: | A testing experiment for the influence of basic media, culture temperature, light intensity, shape of single bud and hormone on in vitro rooting of Camellia oleifera Cenran 2 clone was carried out and the results showed ： 1/2 ER was better than 1/2 WPM and 1/2MS ; ABT6 0. 5mg/L ＋ IAA 2.0 mg/L ＋ NAA0.8 mg/L was the best hormones suitable for the rooting with the percentage up to 93.7% and after 40 days. there were three to four roots in a single bud. The single bud rooted and grew well under the condition of 28% ± 2℃ and 2 500 -3 500 Lx, the rooting percentage was to 97.8 %. |
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| Keywords: | Camellia oleifera tissue culture rooting |
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