| 四种淡水鱼的RAPD分析 |
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| 引用本文： | 沈怀舜,许璞,丁亚平,尹敏敏,胡兆莉,浦佩,陈清兰.四种淡水鱼的RAPD分析[J].水产养殖,2002(2):30-32. |
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| 作者姓名： | 沈怀舜 许璞 丁亚平 尹敏敏 胡兆莉 浦佩 陈清兰 |
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| 作者单位： | 1. 江苏省海洋水产研究所，南通 226007
2. 苏州大学水产学院，苏州 215151 |
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| 摘 要： | 用RAPD技术分析了四种淡水鱼-鲫鱼、鳊鱼、草鱼及鲢鱼的种间和种内的遗传变异。用30个随机引物进行了RAPD扩增，其中29个引物得到扩增产物，选取其中11个带纹清晰、多态性较丰富的引物的扩增图谱进行遗传距离的计算，并进行聚类分析，同时获得四种淡水鱼各自的特异性标记。
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| 关 键 词： | 分子标记 遗传距离 聚类分析 淡水鱼 RAPD分析 基因多态性 |
| 修稿时间： | 2002年2月9日 |
RAPD Analysis on Four kinds of Fresh Water Fish |
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| Shen Huaishun,Xu Pu,Ding Yaping.RAPD Analysis on Four kinds of Fresh Water Fish[J].Journal of Aquaculture,2002(2):30-32. |
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| Authors: | Shen Huaishun Xu Pu Ding Yaping |
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| Abstract: | Intra - and inter - population genetic variation of four kinds of fresh water fish was analysed by using randomamplified polymorphic (RAPD) markers. 29 primers got amplified bands in total of 30 primers, amplified bands of 11primers were selected to statistic similarity index and genetic distances. Cluster analysis based on the RAPD results wasperformed by UPGMA method. Character bands of four kinds of fish were got, these may be useful in germplasm iden-tification of four kinds of fish. |
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| Keywords: | molecuhr marker BAPD genetic distance chser analysis |
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