| 短毛切梢小蠹微卫星富集文库的构建与分析 |
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| 引用本文： | 曹荟,朱家颖,杨璞,赵宁,杨斌.短毛切梢小蠹微卫星富集文库的构建与分析[J].西南林学院学报,2012(5):62-65. |
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| 作者姓名： | 曹荟 朱家颖 杨璞 赵宁 杨斌 |
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| 作者单位： | [1]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]中国林业科学研究院资源昆虫研究所,云南昆明650224 |
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| 基金项目： | 基金项目：西南林业大学科学研究基金面上项目（111128）资助;林业公益性行业科研专项（201004067）资助. |
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| 摘 要： | 采用生物素-磁珠富集法，构建短毛切梢小蠹微卫星富集文库。在该文库中随机挑选100个克隆作测序分析，结果表明：具微卫星位点的克隆占91％；具有10次以上碱基重复次数的微卫星序列占36％，其中最高碱基重复次数为63次；在这些微卫星序列中，完美型占47．3％，非完美型占24．2％，混合型占16．5％。表明获得的微卫星文库是高质量的文库，完美型的比例高于不完美型和混合型，与真核生物微卫星序列特征相符。
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| 关 键 词： | 短毛切梢小蠹 微卫星 文库构建 序列分析 |
Construction and Analysis of Enriched Microsatellite Library of Tomicus brevipilosus |
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| CAO Hui,ZHU Jia-ying,YANG Pu,ZHAO Ning,YANG Bin.Construction and Analysis of Enriched Microsatellite Library of Tomicus brevipilosus[J].Journal of Southwest Forestry College,2012(5):62-65. |
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| Authors: | CAO Hui ZHU Jia-ying YANG Pu ZHAO Ning YANG Bin |
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| Institution: | 1. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming Yunnan 650224, China; 2. Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming Yunnan 650224, China) |
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| Abstract: | The enriched microsatellite library of Tomicus brevipilosus （Eggers） was constructed using biotin-magnetic bead enrichment method. One hundred clones randomly chosen from the library were sequenced. The results showed that 91% of microsateUite loci were obtained. Among which the microsatellite sequences with more than 10 times of alkaline base repetitions accounted for 36%, and the highest alkaline base repetition was 63 times. From these microsatellites, 47.3% （about 43 repeat motifs） were perfect, 24.2% （about 22 repeat motifs） were imperfect, and 16.5% （about 15 repeat motifs） were compound. The results indicated that the enriched microsatellite library was of high-quality, in which the proportion of perfect microsatellites types was higher than that of the imperfect and compound types, which was in accordance with the feature of microsatellite sequences of other eukaryote. |
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| Keywords: | Tomicus brevipilosus microsatellite library construction sequence analysis |
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