| 三七根际链霉菌Streptomyces sp. YNUCC0233木聚糖酶性质研究 |
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| 引用本文： | 廖昌珑,李文鹏,刘士清,王启方.三七根际链霉菌Streptomyces sp. YNUCC0233木聚糖酶性质研究[J].土壤通报,2005,36(4):591-594. |
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| 作者姓名： | 廖昌珑 李文鹏 刘士清 王启方 |
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| 作者单位： | 1. 云南大学,工业微生物发酵工程重点实验室,云南,昆明,650091
2. 云南大学,工业微生物发酵工程重点实验室,云南,昆明,650091;云南师范大学,农村能源工程重点实验室,云南,昆明,650092 |
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| 基金项目： | 云南省科技计划项目(2001GG24) |
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| 摘 要： | 从三七根际分离到一株产木聚糖酶链霉菌Streptomycessp.YNUCC0233。该菌所产生的木聚糖酶的最适反应温度为67℃,最适pH为6.0,在pH5.0~9.0和60℃以下时相对稳定。Ba2+和K+对该木聚糖酶有较强的促进作用,Hg2+、Cu2+、Mn2+、Co2+、Ni2+和EDTA对该酶有较强的抑制作用。对Streptomycessp.YNUCC023316SrDNA的1105bp片段的序列分析结果表明,Streptomycessp.YNUCC0233的16SrDNA片段与GenBank中所有已注册的链霉菌分类单位均不同。
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| 关 键 词： | 三七 根际 链霉菌YNUCC0233 木聚糖酶 16SrDNA |
| 文章编号： | 0564-3945(2005)04-0591-04 |
| 修稿时间： | 2004年3月16日 |
Isolation and Characterization of Xylan-degrading Streptomyces sp.YNUCC0233 Associated with the Rhizosphere of Panax notoginseng (Burk.) F.H.Chen |
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| LIAO Chang-long,LI Wen-peng,LIU Shi-qing,WANG Qi-fang.Isolation and Characterization of Xylan-degrading Streptomyces sp.YNUCC0233 Associated with the Rhizosphere of Panax notoginseng (Burk.) F.H.Chen[J].Chinese Journal of Soil Science,2005,36(4):591-594. |
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| Authors: | LIAO Chang-long LI Wen-peng LIU Shi-qing WANG Qi-fang |
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| Institution: | LIAO Chang-long~1,LI Wen-peng~ |
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| Abstract: | A strain namely Streptomyces sp.YNUCC0233 was isolated from the rhizosphere of Panax notoginseng (Burk.) F.H.Chen. The strain YNUCC0233 produced a maximum amount of xylanase (120 UmL-1) at 28 for 5 days. The crude xylanase exhibited the highest activity at pH6.00 and at the temperature 67. Xylanase was stable at an abroad pH range between pH 5.0 ~ pH 9.0 and under the temperature 60. Hg~(2 ),Cu~(2 ),Mn~(2 ) Co~(2 ),Ni~(2 ) and EDTA showed strong inhibition on the activity of the xylanase, while K~ and Ba~(2 ) born a remarkable promotion on the enzyme activity. A 1105bp long fragment of 16S rDNA from Streptomyces sp.YNUCC0233 was sequenced and compared with closely related 19 taxon in GenBank. The phylogenetic tree constructed by Neighbor-joining method indicated that fragment of 16S rDNA from Streptomyces sp.YNUCC0233 was unique in GenBank. |
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| Keywords: | Panax notoginseng Rhizosphere Streptomyces sp YNUCC0233 Xylanase 16S ribosomal DNA |
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