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| 克氏原螯虾细菌性病原的分离与鉴定 | |
| 引用本文： | 周冬仁,章文敏,李建应,施伟达,罗毅志,叶雪平.克氏原螯虾细菌性病原的分离与鉴定[J].中国农学通报,2011,27(26):102-105. |
| 作者姓名： | 周冬仁 章文敏 李建应 施伟达 罗毅志 叶雪平 |
| 作者单位： | 1. 浙江省淡水水产研究所,浙江湖州,313001 2. 安吉县水产推广站,浙江湖州,313000 3. 德清县水产推广站,浙江湖州,313000 |
| 基金项目： | 浙江省优选主题重大农业项目"急性出血性综合症病原、发病机理及控制技术研究" |
| 摘 要： | 2010年浙江安吉克氏原螯虾养殖发生严重病害，从患病的虾肠道内分离到菌株N201007，人工感染健康克氏原螯虾在8天内其半数致死量LD50为5.3×105，感染发病虾表现出与自然发病虾相似症状。通过革兰氏染色，梅里埃细菌测定试剂条ID32 E鉴定为嗜水气单胞菌。为进一步确定病原菌分类地位，对其16S rRNA序列扩增并测序，在NCBI基因库上进行序列比对，与菌株ATCC7699同源性达到99%，通过以上实验确定导致此次养殖克氏原螯虾大规模死亡的病原为嗜水气单胞菌。药敏试验结果表明该菌对强力霉素、氯霉素、恩诺沙星、环丙沙星、菌必治敏感。 |
| 关 键 词： | 木薯 低温胁迫 相对电导率 丙二醛 脯氨酸 木薯 低温胁迫 相对电导率 丙二醛 脯氨酸 |
| 收稿时间： | 3/2/2011 12:00:00 AM |
| 修稿时间： | 2011/3/16 0:00:00 |
Isolation and Identification of bacterial pathogen from Procambarus clarkii |
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| Zhou Dongren,Zhang Wenmin,Li Jianying,Shi Weida,Luo YiZHi,Ye Xueping.Isolation and Identification of bacterial pathogen from Procambarus clarkii[J].Chinese Agricultural Science Bulletin,2011,27(26):102-105. | |
| Authors: | Zhou Dongren Zhang Wenmin Li Jianying Shi Weida Luo YiZHi Ye Xueping |
| Institution: | Zhou Dongren 1,Zhang Wenmin 2,Li Jianying 3,Shi Weida 1,Luo YiZHi 1,Ye Xueping 1 (1 Zhejiang Institute of Freshwater Fisheries,Huzhou Zhejiang 313001,2 Anji Fisheries Technical ExtensionStation,Huzhou Zhejiang 313000,3 Deqing Fisheries Technical Extension Station,Huzhou Zhejiang 313000) |
| Abstract: | One bacterial strain of Aeramonas hydrophila （N201007） was isolated from affected Procambarus clarkii in Anji area Zhejiang Province during an outbreak in 2010.The isolate could induce symptom of natural disease in ornamental Procambarus clarkii and caused death in 8 days,The semi-lethal LD 50 to Procambarus clarkii was 5.3×10 5.Biological characterization showed that isolate was similar to A.hydrophila isolated in other aquatic animal.Analysis of 16S rRNA gene showed that the isolate shared more than 99% identity with A.hydrophila.Based on morphological,biochemical and phylogenetic analysis,the isolate was identified as A.hydrophila.The antibiotic sensitivity test showed N201007 was hypersensitive to doxycycline,chloramphenicol,enrofloxacin,ciprofloxacin and rocephin. |
| Keywords: | Procambarus clarkia Aeromonas hydrophila biochemical characteristic drug sensitivity test |
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