| 亚洲百合试管苗生根的研究 |
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| 引用本文： | 刘婕,刘雅莉,贾敏,王岩.亚洲百合试管苗生根的研究[J].中国农学通报,2011,27(28):163-166. |
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| 作者姓名： | 刘婕 刘雅莉 贾敏 王岩 |
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| 作者单位： | 旱区作物逆境生物学国家重点实验室/西北农林科技大学林学院,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金项目"百合ACC合成酶反义基因构建及其遗传转化" |
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| 摘 要： | 为了优化亚洲百合生根培养基配方，为亚洲百合转基因植株试管苗的生根移栽奠定一定的基础，以亚洲百合‘普利安娜’（Lilium Asiatic hybrid cv. ‘Pollyanna’）和‘普瑞头’（Lilium Asiatic hybrid cv. ‘Prato’）试管苗为材料，探讨5种不同激素的浓度组合对试管苗生根率、生根数量、最长根长的影响。结果表明：不同植物激素的组合和浓度对亚洲百合的生根有显著影响，且2个品种生根状况有一定的差异。在生根培养基为MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂7 g/L时，亚洲百合‘普利安娜’平均生根率达到98.73%，生根数量达到6.53条，最长根长为4 cm；生根培养基为：MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂7 g/L时，亚洲百合‘普瑞头’平均生根率达到92.73%，生根数量达到6.63条，最长根长为3.77 cm。最后得出：亚洲百合‘普利安娜’最适宜的生根培养基为：MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂7 g/L；亚洲百合‘普瑞头’的适宜生根培养基为：MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂7 g/L。
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| 关 键 词： | 亚洲百合 植物激素 生根 |
| 收稿时间： | 2011/6/20 0:00:00 |
| 修稿时间： | 8/4/2011 12:00:00 AM |
Research on Rooting of Asian Lily Plantlets |
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| Liu Jie,Liu Yali,Jia Min,Wang Yan.Research on Rooting of Asian Lily Plantlets[J].Chinese Agricultural Science Bulletin,2011,27(28):163-166. |
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| Authors: | Liu Jie Liu Yali Jia Min Wang Yan |
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| Institution: | Liu Jie,Liu Yali,Jia Min,Wang Yan(State Key Laboratory of Crop Stress Biology in Arid Areas/College of Forestry,Northwest A &F University,Yangling Shaanxi 712100) |
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| Abstract: | In order to optimize the formulation of Asian lily rooting medium and lay a foundation for rooting and transplanting of Asian lily transgenic plants,Lilium Asiatic hybrid cv.' Pollyanna ' and Lilium Asiatic hybrid cv.' Prato ' test-tube seedlings were used to study the effect of combinations of five different hormone in different concentrations on rooting rate,root number,longest root length.The results showed that:a combination of different types and concentrations of plant hormones significantly affected ... |
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| Keywords: | Asian lily plant hormone rooting |
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