Biodegradation of 3-chloro-1,2-propanediol with Saccharomyces cerevisiae |
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| Authors: | Bel-Rhlid Rachid Talmon Jean P Fay Laurent B Juillerat Marcel A |
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| Institution: | Nestlé Research Center, Nestec Ltd., Vers-Chez-les-Blanc, P.O. Box 44, 1000 Lausanne 26, Switzerland. |
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| Abstract: | A novel enzymatic dehalogenating activity of 3-chloro-1,2-propanediol (3-MCPD) with Saccharomyces cerevisiae (baker's yeast) is reported. All bioconversion assays were carried out under aerobic conditions, at 28 degrees C, and the kinetics were monitored. The biodegradation was performed at different pH values (6.2, 7.0, and 8.2), in the presence and absence of glucose, using racemic 3-MCPD at two different concentrations (7.3 micromol/L and 27 mmol/L). Optimal conversion (68%) of racemic (R,S)-3-MCPD at a concentration of 27 mmol/L was achieved after 48 h of reaction time, at pH 8.2, and in the presence of glucose. At a concentration of 7.3 micromol/L, 73% degradation was observed after 72 h, at pH 8.2 and in the absence of glucose. Under the same experimental conditions, the conversion of pure (S)-3-MCPD (85%) was higher than that of the (R)-enantiomer (60%). |
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