| 枯草芽胞杆菌BS501a复合诱变菌株及抗菌谱研究 |
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| 引用本文： | 李瑞芳,赵玉峰,杨世清,伊艳杰,田泱源.枯草芽胞杆菌BS501a复合诱变菌株及抗菌谱研究[J].植物保护,2011,37(5):86-91. |
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| 作者姓名： | 李瑞芳 赵玉峰 杨世清 伊艳杰 田泱源 |
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| 作者单位： | 1. 河南工业大学生物工程学院,郑州,450001
2. 河南省科学院同位素研究所有限公司,郑州,450052 |
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| 摘 要： | ［目的］ 通过复合诱变进一步提高枯草芽胞杆菌BS501a的生防效果。［方法］ 以BS501a为出发菌株，采用N+离子注入和60Coγ射线照射进行复合诱变。［结果］ 获得了5株拮抗活性提高的突变菌株。传代稳定性试验表明BSC45菌株经5次传代后拮抗稳定性仍保持在88.3%。该突变株各代与稻瘟病菌对峙培养拮抗带宽平均为7.5 mm，比出发菌株BS501a与稻瘟病菌对峙培养拮抗带宽6.05 mm提高了24%。采用对峙培养试验研究BSC45抗菌谱，发现BSC45仍保留了广谱拮抗活性。［结论］ 枯草芽胞杆菌突变株BSC45对多种真菌有抗菌作用，是一株很有潜力的生防菌株。
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| 关 键 词： | N+离子注入 60Co γ射线辐照 复合诱变 拮抗活性 抗菌谱 |
Multiplex mutation isolates of Bacillus subtilis BS501a and the antimicrobial spectrum |
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| Li Ruifang,Zhao Yufeng,Yang Shiqing,Yi Yanjie,Tian Yangyuan.Multiplex mutation isolates of Bacillus subtilis BS501a and the antimicrobial spectrum[J].Plant Protection,2011,37(5):86-91. |
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| Authors: | Li Ruifang Zhao Yufeng Yang Shiqing Yi Yanjie Tian Yangyuan |
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| Institution: | 1. School of Bioengineering, Henan University of Technology, Zhengzhou450001, China; 2. Institute of Isotopes Co. Ltd., Henan Academy of Sciences, Zhengzhou450052, China |
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| Abstract: | ［Objective］ To promote the biological control effects of Bacillus subtilis BS501a by multiplex mutation. ［Method］ The initial antagonistic strain BS501a was treated by N+ ions implantation and 60Co γ irradiation. ［Result］ Five mutants were screened after multiplex mutation. Genetic stability of the mutant BSC45 remained to be 88.3% after being cultured for 5 generations. The average width of inhibition zones of the mutants against Magnaporthe grisea was 7.5 mm. There was a 1.24 fold increase as compared with the control strain BS501a. The antimicrobial spectrum of BSC45 was studied using antagonistic experiments. The results showed that the mutant BSC45 had a broad spectrum antagonistic activity. ［Conclusion］ The isolate Bacillus subtilis BSC45, with antagonistic activity to many different fungi, was a promising biocontrol isolate. |
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| Keywords: | N+ ion implantation 60Co γ irradiation multiplex mutation antagonistic activity antimicrobial spectrum |
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