Myc-induced T cell leukemia in transgenic zebrafish |
| |
| Authors: | Langenau David M Traver David Ferrando Adolfo A Kutok Jeffery L Aster Jon C Kanki John P Lin Shuo Prochownik Ed Trede Nikolaus S Zon Leonard I Look A Thomas |
| |
| Institution: | Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. |
| |
| Abstract: | The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-myc under control of the zebrafish Rag2 promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
