| 不同磷质量分数下马尾松幼苗根的生理生化特征 |
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| 引用本文： | 刘娅惠,徐瑾,雷蕾,万一,曾立雄,肖文发.不同磷质量分数下马尾松幼苗根的生理生化特征[J].浙江农林大学学报,2023,40(1):126-134. |
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| 作者姓名： | 刘娅惠 徐瑾 雷蕾 万一 曾立雄 肖文发 |
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| 作者单位： | 1.中国林业科学研究院 森林生态环境与自然保护研究所 国家林业和草原局森林生态环境重点实验室，北京 1000912.南京林业大学 南方现代林业协同创新中心，江苏 南京 210037 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(CAFYBB2021QD002) |
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| 摘 要： | 目的 研究不同质量分数度下马尾松Pinus massoniana幼苗根系生理生化特征，以探究马尾松应对磷胁迫的生理生化机制，为低磷区域的马尾松人工林经营提供理论支持。 方法 以2年生马尾松幼苗为材料，采用石英砂盆栽的方法，基于全国马尾松林土壤有效磷质量分数(2.250 0 mg·kg?1)，设计0 (P0)、0.562 5 (P1)、1.125 0 (P2)、2.250 0 (ck)、4.500 0 (P3)、9.000 0 (P4) mg·kg?1共6个有效质量分数度，分析了2个采样时间8月(处理1.5个月)与9月(处理3.0个月后)]马尾松幼苗根系活力及酸性磷酸酶(ACP)活性、有机酸质量摩尔浓度、丙二醛(MDA)质量摩尔浓度以及3种抗氧化保护酶活性超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)]的差异。 结果 ①随着磷质量分数的增加，ACP活性与有机酸质量摩尔浓度均呈下降趋势。其中，P0处理的ACP活性在8和9月均显著高于ck (P＜0.05)，P4处理的ACP活性在8和9月均显著低于ck (P＜0.05)；在9月，P3处理的ACP活性也显著低于ck (P＜0.05)；在2个采样时间段，P0和P1处理的有机酸质量摩尔浓度显著高于ck (P＜0.05)，P4处理的有机酸质量摩尔浓度显著低于ck (P＜0.05)。②8月ck的根系活力显著低于P0、P1、P3、P4处理(P＜0.05)，而9月根系活力随磷质量分数的增加而增加。③MDA随着磷质量分数的降低而增加，P0处理显著高于ck (P＜0.05)，P4处理则显著低于ck (P＜0.05)。仅P0和P1处理的3种抗氧化保护酶活性处理显著高于ck (P＜0.05)。 结论 低磷胁迫会使马尾松幼苗根系MAD增加并影响其根系活力，但是马尾松幼苗通过增加根ACP活性与有机酸质量摩尔浓度以及抗氧化系统酶活性来应对低磷胁迫的影响。图3表1参39
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| 关 键 词： | 马尾松幼苗 磷 根系活力 抗氧化保护酶 |
| 收稿时间： | 2022-03-17 |
Physiological and biochemical responses of seedling roots of Pinus massoniana to different phosphorus concentrations |
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| LIU Yahui,XU Jin,LEI Lei,WAN Yi,ZENG Lixiong,XIAO Wenfa.Physiological and biochemical responses of seedling roots of Pinus massoniana to different phosphorus concentrations[J].Journal of Zhejiang A&F University,2023,40(1):126-134. |
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| Authors: | LIU Yahui XU Jin LEI Lei WAN Yi ZENG Lixiong XIAO Wenfa |
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| Institution: | 1.Key Laboratory of Forest Ecology and Environment, National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China2.Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, Jiangsu, China |
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| Abstract: | Objective This study, with an investigation of the physiological and biochemical responses of roots in Pinus massoniana seedlings to different phosphorus (P) concentrations, is aimed to explore the physiological and biochemical mechanisms of P. massoniana in response to low P stress, so as to provide theoretical support for the managements of P. massoniana plantations in low phosphorus area. Method First, two-year-old seedlings of P. massoniana planted in potted with quartz sand were treated with six available P concentrations, including 0 (P0)、0.562 5 (P1)、1.125 0 (P2)、2.250 0 (ck)、4.500 0 (P3)、9.000 0 (P4) mg·kg?1 before a control check (ck, 2.250 0 mg·kg?1) with the effective phosphorus content of the national P. massoniana forest soil survey. Then, the physiological and biochemical characteristics of roots, including activities of acid phosphatase (ACP), total organic acid content (TOC), root vigor, malondialdehyde (MDA) and activities of three antioxidant protective enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), were determined and analyzed in both 1.5 and 3.0 months after treatments. Result (1) Both ACP activity and organic acid content decreased with the increase of phosphorus concentration with the ACP activity of P0 in August and September being significantly higher than that of ck (P＜0.05), and the ACP activity of P4 being significantly lower than ck both in August and September (P＜0.05) whereas in September, the ACP activity of P3 was also significantly lower than that of ck (P＜0.05) with the organic acid content in P0 and P1, and organic acid content in P4 being significantly lower than in ck (P＜0.05) in two sampling times. (2) In August, the root vigor in ck was significantly lower than in P0, P1, P3 and P4 (P＜0.05), and in September, the root vigor increased with the increase of phosphorus concentration. (3) The root MDA content increased with the decrease of phosphorus concentration with the MDA content in P0 being significantly higher than that in ck (P＜0.05), and the MDA content in P4 being significantly lower than that in ck (P＜0.05). The antioxidant enzyme activities showed a tendency of increasing as phosphorus concentration decreased whereas SOD, POD and CAT were significantly higher in P0 and P1 than that in ck (P＜0.05) though their activities showed no significant variation among ck, P2, P3 and P4 treatments. Conclusion In response to low P stress, P. massoniana seedlings had their MAD, ACP, TOC and antioxidant system enzyme activities increased in roots with the root vigor regulated. Ch, 3 fig. 1 tab. 39 ref.] |
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