| 白菜黑斑病菌拮抗细菌LBC-1菌株的分离与鉴定 |
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| 引用本文： | 李春辉,李术娜,王纳贤,杜淑涛,王世英,朱宝成.白菜黑斑病菌拮抗细菌LBC-1菌株的分离与鉴定[J].中国蔬菜,2009,1(20):29-34. |
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| 作者姓名： | 李春辉 李术娜 王纳贤 杜淑涛 王世英 朱宝成 |
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| 作者单位： | 1.河北大学生命科学学院，河北保定 071002；2河北农业大学生命科学学院，河北保定 071001 |
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| 摘 要： | 从湖南、湖北、河北等地区菜地采集土样，采用抑菌圈法筛选对白菜黑斑病病原菌芸薹链格孢菌（Alternaria brassicae）具有拮抗作用的芽孢杆菌菌株。通过初筛得到具有拮抗作用的菌株20株，复筛出1株拮抗活性较高的菌株LBC-1。通过生理生化试验、形态特征观察及16S rDNA序列分析对其进行了种属鉴定。鉴定结果为菌株LBC-1属于解淀粉芽孢杆菌（Bacillus amyloliquefaciens），是1株广谱拮抗芽孢细菌菌株。
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| 关 键 词： | 拮抗细菌 筛选 鉴定 解淀粉芽孢杆菌 |
| 收稿时间： | 2009-02-26; |
Separation and Identification of Bacillus LBC-1 against Alternaria brassicae |
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| LI Chun-hui,LI Shu-na,WANG Na-xian,DU Shu-tao,WANG Shi-ying,ZHU Bao-cheng.Separation and Identification of Bacillus LBC-1 against Alternaria brassicae[J].China Vegetables,2009,1(20):29-34. |
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| Authors: | LI Chun-hui LI Shu-na WANG Na-xian DU Shu-tao WANG Shi-ying ZHU Bao-cheng |
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| Institution: | 1.College of Life Science,Hebei University,Baoding 071002,Hebei,China;2.College of Life Science,Hebei Agricultural University,Baoding 071001,Hebei,China |
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| Abstract: | Twenty strains of Bacillus were isolated by sampling soil from fields of various regions via preliminary screening.They have antagonistic activities against Alternaria brassicae.A strain named LBC-1 with a relatively higher antagonistic activity was obtained via secondary screening.Strain LBC-1 was characterized by physiological and biochemical experiments,morphological features observation,and 16S rDNA sequence analysis.Based on the results,strain LBC-1 was finally identified as Bacillus amyloliquefaciens.Strain LBC-1 was a broad-spectrum antagonistic strains of spore-forming bacteria. |
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