| 稻瘟病菌不同小种菌株间无性重组的研究 |
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| 引用本文： | 张正光,郑小波.稻瘟病菌不同小种菌株间无性重组的研究[J].植物病理学报,2000,30(4):312-318. |
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| 作者姓名： | 张正光 郑小波 |
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| 作者单位： | 南京农业大学植保系农业部病虫监测与治理重点开放实验室, 南京 210095 |
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| 基金项目： | 江苏省“九五”重大攻关项目! (GB96 5 12 - 1)，国家自然科学基金! (39970 0 2 6 )，教育部跨世纪优秀人才培养计划基金资助 |
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| 摘 要： | 将分别携带抗稻瘟灵对多菌灵敏感(MBCsIPTr)标记与携带抗多菌灵对稻瘟灵敏感(MBCrIPTs)标记的稻瘟病菌营养体亲和的不同小种菌株在白米玫瑰红培养基上,25℃、黑暗中对峙培养18d,配对菌株菌落交界处出现肉眼可见的菌丝融合带。4个小种间营养体可亲和菌株配对组合是:PO21-MBCr-27(ZA49)×PO3-IPTr-54(ZF1)、PO21-MBCr-27(ZA49)×PO2-IPTr-51(ZF1)、PO21-MBCr-27(ZA49)×PO33-IPTr-69(ZD1)和PO21-MBCr-41(ZA49)×PO3-IPTr-54(ZF1)。分别切取各配对组合产生的菌丝融合带,分别建立单菌丝片段群体,测定其对稻瘟灵、多菌灵的抗药性。结果是,从4个配对组合的单菌丝片段后代中,均检测到分别携带MBCsIPTr、MBCrIPTs标记和同时携带双亲抗性标记(MBCrIPTr)3种个体,其中MBCrIPTr个体的检出率为1.0%~3.6%。无性重组体的MBCr和IPTr性状在其菌丝块移植继代培养和单菌丝片段株后代发生分离。以配对组合PO21-MBCr-27×PO3-IPTr-54产生的菌丝融合带建立单孢无性系,从3178个单孢株中筛选到4株MBCrIPTr个体,其MBCrIPTr性状在单孢后代和单芽管分离株后代均能稳定遗传。结果表明,营养体亲和的稻瘟病菌不同小种菌株之间可通过菌丝融合产生无性重组体,同时提示无性重组体内可能发生了准性生殖。
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| 关 键 词： | 稻瘟病菌 不同小种菌株 无性重组 |
| 修稿时间： | 1999-09-07 |
INTER RACIAL ASEXUAL RECOMBINATION IN Magnaporthe grisea |
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| ZHANG Zheng guang,ZHENG Xiao bo.INTER RACIAL ASEXUAL RECOMBINATION IN Magnaporthe grisea[J].Acta Phytopathologica Sinica,2000,30(4):312-318. |
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| Authors: | ZHANG Zheng guang ZHENG Xiao bo |
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| Institution: | The Key Lab of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture, Dept. of Plant Protection, Nanjing Agric. Univ., Nanjing 210095, China |
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| Abstract: | Four wild type isolates of Magnaporthe grisea from three vegetative compatible pai rings, PO21(race ZA49)×PO2(race ZF1),PO21(race ZA49)×PO3(race ZF1) and PO21(race ZA49)×PO33(race ZD1) which formed a solid hyphal fusion line between the colonies when paired on polished rice rose bengal medium, were induced fungicide resistance in vitro. The chemical resistance in the isoprothiolane resistance (IPTr) mutant PO3 IPTr, PO2 IPTr, PO33 IPTr, and carbendazin resistance (MBCr) mutant PO21 MBCr inherited stably in two successive conidium progenies. On the contrary, the IPTr mutants selected were sensitive to MBC (MBC s) and the MBCr mutant was sensitive to IPT (IPTs) in the successive conidial progenies. Four vegetative compatible monoconidial isolate pairings with different chemical resistant markers,PO21 MBCrIPTs 27(race ZA49)×PO3 MBC sIPTr 54(race ZF1),PO21 MBCrIPTs 41(race ZA49)×PO3 MBC sIPTr 54(race ZF1),PO21 MBCrIPTs 27(race ZA49)×PO2 MBC sIPTr 51(race ZF1) and PO21 MBCrIPTs 27(race ZA49)×PO33 MBC sIPTr 69(race ZD1) were selected. The asexual recombinants with marker MBCrIPTr were detected in all of the four pairings in their single hyphal fragment progenies established respectively from the hyphal fusion lines presented between the paired inoculated isolates opposite on polished rice rose bengal medium and incubated at 25±1℃ in darkness for 18 days. The percentage of recombinants detected was about 1.0%-3.6% (in about 200 single hyphal fragment isolates tested for each pairing). However, the marker MBCrIPTr in recombinants separated obviously in further hyphal fragment progenies even in the subcultures by mass transfers. In this study, four monoconidial isolates carried MBCrIPTr marker were also selected out from 3178 monoconidium isolates from hyphal fusion line presented in the pairing PO21 MBCrIPTs 27(race ZA49)×PO3 MBC sIPTr 54(race ZF1), but the marker MBCrIPTr was stably inherited in their monospore and single germ tube progenies. The results indicated that asexual recombination by anastomosis between races and parasexuality occurred in M. grisea. It suggested that asexual recombination may be an important mechanism for M. grisea to produce and maintain genetic diversity in wild population. |
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| Keywords: | Magnaporthe grisea inter race asexual recombination |
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