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采用16S rDNA鉴定甜瓜细菌性叶斑病菌
引用本文:李亚利,贾迎春,蒲崇建,杨成德,陈秀蓉.采用16S rDNA鉴定甜瓜细菌性叶斑病菌[J].植物保护,2007,33(3):65-68.
作者姓名:李亚利  贾迎春  蒲崇建  杨成德  陈秀蓉
作者单位:1.甘肃农业大学草业学院,兰州 730070;2.甘肃省植保植检总站,兰州 730030
摘    要:从甘肃河西、新疆阿勒泰甜瓜上分离获得的2株致病细菌,通过16S rDNA序列测定以及序列同源性比较,结合病原菌落培养性状、菌体形态观察和革兰氏染色反应等,初步确定当地甜瓜细菌性叶斑病菌为丁香假单胞杆菌Pseudomonas syringae pv.lachrymans (Smith et Bryan) Young et al.]

关 键 词:细菌性叶斑病  致病性测定  16S  rDNA鉴定

Identification of the pathogen of bacterial spot of cucurbits leaves based on 16S rDNA sequences
Li Yali,Jia Yingchun,Pu Chongjian,Yang Chengde,Chen Xiurong.Identification of the pathogen of bacterial spot of cucurbits leaves based on 16S rDNA sequences[J].Plant Protection,2007,33(3):65-68.
Authors:Li Yali  Jia Yingchun  Pu Chongjian  Yang Chengde  Chen Xiurong
Institution:1.College of Pratacultural Science, Gansu Agricultural University, Lanzhou 730070, China; 2. Plant Protection and Quarantine Station of Gansu, Lanzhou 730070, China
Abstract:Two strains of pathogenic bacteria were isolated in Hexi in Gansu and Aletai in Xinjiang.The strains were examined using 16S rDNA gene sequences.The results showed that the pathogens might be Pseudomonas syringae pv.lachrymans(Smith et Bryan) Young et al.,based on their culture patterns,morphological characteristics and staining reactions.
Keywords:

bacterial leave spot  pathogenicity  identification based on 16S rDNA

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