| 利用RAPD技术对5个油菜(B.napus)品种进行鉴别 |
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| 引用本文： | 王学军,熊兴华,官春云.利用RAPD技术对5个油菜(B.napus)品种进行鉴别[J].作物研究,2010,24(2):99-102. |
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| 作者姓名： | 王学军 熊兴华 官春云 |
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| 作者单位： | 湖南农业大学,长沙,410128 |
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| 基金项目： | 湖南农业大学人才基金，湖南省科技厅项目 |
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| 摘 要： | 油菜品种的分子鉴别在油菜分子育种和种质资源管理中有重要意义。利用RAPD分子标记技术对5个双低油菜品种或杂种(湘油15号、湘油11号、湘农油571、杂695、杂814)进行品种鉴定,建立了适合油菜的RAPD反应体系:30μL反应体系中含50 ng模板,40 pmol/L引物,150μmol/L dNTP,1.5 UTaq DNA Polymerase,2.5 mmol/L Mg2+,35.5℃的退火温度。找到了可快速鉴别5个油菜品种或杂种的两条随机引物(S151和S369),为这几个油菜品种的分子鉴别找到依据。
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| 关 键 词： | 油菜 RAPD 品种鉴定 分子标记 |
Identification of Five Rapeseed (B.napus) Varieties with RAPD Technique |
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| WANG Xue-jun,XIONG Xing-hua,GUAN Chun-yun.Identification of Five Rapeseed (B.napus) Varieties with RAPD Technique[J].Crop Research,2010,24(2):99-102. |
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| Authors: | WANG Xue-jun XIONG Xing-hua GUAN Chun-yun |
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| Institution: | (Hunan Agricultural University, Changsha, Hunan 410128, China) |
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| Abstract: | Molecular identification of rapeseed varieties has important significance in the molecular breeding and germplasm resource management of rapeseed. Five double low rapeseed varieties, i. e. Xiangyou No. 15, XiangYou No. 11, Xiangnongyou 571, Za695 and Za814, have been identified by RAPD technique. The optimal RAPD reaction conditions for rapeseed were 50 ng DNA and 40 pmol/L primer, 150 μmol/L dNTP, 1.5 UTaq DNA polymerase, 2.5 mmol/L Mg^2＋ in 30 μL reaction system. Two random primers, S151 and S369, could used to effectively and quickly identify the five rapeseed cultivars, which provided the basis for molecular identification of the tested rapeseed varieties. |
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| Keywords: | RAPD |
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