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DETERMINATION OF OLIVE MILL WASTEWATER TOXIC EFFECTS ON THREE MINT SPECIES GROWN IN HYDROPONIC CULTURE
Authors:Georgia Ouzounidou  Maria Asfi
Institution:1. Institute of Food Technology, National Agricultural Research Foundation , Lycovrissi , Greece;2. Department of Botany , Aristotle University of Thessaloniki , Thessaloniki , Greece
Abstract:The effects of two olive mill wastewater (OMW) dilutions upon physiological function and metabolic components of three mint species grown in hydroponic culture were studied. Visible symptoms, chlorosis and stunted growth, were pronounced in peppermint and spearmint; toxicity symptoms in menthe douce were few. Peppermint and spearmint displayed loss of calcium (Ca), magnesium (Mg), and potassium (K); menthe douce revealed accumulation of Ca, sodium (Na), and iron (Fe), under OMW. The loss of Ca negatively affected the development and stability of cell wall and membrane; the Mg deficiency negatively affected the photosynthetic apparatus and carbon fixation. Fv′/ Fm′ was slightly affected under OMW, whereas, ΦPSII, q P and Rfd displayed significant reductions. No clear effect of OMW on phenols and carbohydrates among the species were found. Ascorbic acid content in peppermint dropped dramatically; the reduction was lower for spearmint and menthe douce. The OMW resistant species, menthe douce, developed a mechanism to control the physiological and the biochemical status.
Keywords:ascorbic acid  carbohydrates  olive mill wastewater  Mentha piperita  M  spicata  M  viridis  photosynthesis  polyphenols
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