| 一株欧洲类禽H1N1猪流感病毒的分离鉴定与反向遗传系统的建立 |
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| 引用本文： | 胡晓鲁,张婷,兰海玲,李雪松,杨健美,滕巧泱,陈鸿军,李泽君,刘芹防,关平原.一株欧洲类禽H1N1猪流感病毒的分离鉴定与反向遗传系统的建立[J].中国动物传染病学报,2019(3):27-32. |
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| 作者姓名： | 胡晓鲁 张婷 兰海玲 李雪松 杨健美 滕巧泱 陈鸿军 李泽君 刘芹防 关平原 |
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| 作者单位： | 内蒙古农业大学兽医学院;中国农业科学院上海兽医研究所;黑龙江大学生命科学学院 |
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| 基金项目： | 国家重点研发计划(2017YFD0500806);国家自然科学基金面上项目(31572543、31772753);中国农业科学院青年英才项目(CAASQNYC-KYYJ-58) |
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| 摘 要： | 猪流感是猪常见的呼吸道传染病,临床以高热、呼吸困难、咳嗽和衰竭、迅速康复或死亡为特征。猪流感不仅给养猪业造成巨大损失,也严重威胁着人类健康。本研究从发病猪场中分离到1株H1N1亚型猪流感病毒,序列分析结果显示,分离毒株属于欧洲类禽猪流感H1N1亚型病毒。将分离毒株分别接种到MDCK与ST细胞,观察病毒的生长特性,结果显示分离的猪流感病毒在ST细胞中复制能力较强。采用RT-PCR技术分别扩增8个基因片段,克隆到流感病毒反向遗传系统,成功拯救出猪流感病毒毒株,测序结果显示拯救的猪流感病毒与亲本毒序列一致。本研究成功分离的猪流感病毒,以及建立的反向遗传技术为研究欧洲类禽猪流感病毒跨种传播的机制以及研发新型猪流感疫苗株奠定了基础。
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| 关 键 词： | 欧洲类禽猪流感病毒 H1N1亚型 反向遗传系统 |
APPLICATION OF REVERSE GENETICS FOR AN H1N1 SWINE INFLUENZA VIRUS |
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| HU Xiao-lu,ZHANG Ting,LAN Hai-ling,LI Xue-song,YANG Jian-mei,TENG Qiao-yang,CHEN Hong-jun,LI Ze-jun,LIU Qin-fang,GUAN Ping-yuan.APPLICATION OF REVERSE GENETICS FOR AN H1N1 SWINE INFLUENZA VIRUS[J].Chinese Journal of Animal Infectious Diseases,2019(3):27-32. |
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| Authors: | HU Xiao-lu ZHANG Ting LAN Hai-ling LI Xue-song YANG Jian-mei TENG Qiao-yang CHEN Hong-jun LI Ze-jun LIU Qin-fang GUAN Ping-yuan |
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| Institution: | (College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018,China;Shanghai Veterinary Research Institute, CAAS, Shanghai 200241, China;College of Bioscience , Heilongjiang University, Harbin 150080,China) |
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| Abstract: | Swine influenza is a common respiratory disease in pigs with clinical signs including labored breathing, nasal discharge,sneezing, coughing and fever. It causes significant economic losses to swine industry and poses a serious threat to human health, which has important public health significance. In this study, an H1 N1 swine influenza virus(SIV) was isolated from a diseased pig and similar to EA H1 N1 SIV according to the evolutionary analysis. This virus showed better proliferation in ST cells than MDCK cells.The entire eight segments of this virus were amplified by RT-PCR and cloned into the reverse genetics system. The rescued SIV was used to transfect 293 T by eight plasmids. The sequence of the rescued virus was the same as the parent virus through complete genome sequencing. The availability of the rescued SIV built a platform for the research of the mechanisms of cross-species transmission of EA H1 N1 SIV and for the development of SIV vaccine. |
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| Keywords: | Swine influenza virus H1N1 reverse genetics |
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