| 淡紫拟青霉M-1固体发酵工艺优化及基于自动化种曲机的中试生产 |
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| 引用本文： | 周莲,谢小林,吴天福,陈美标,姚青,朱红惠.淡紫拟青霉M-1固体发酵工艺优化及基于自动化种曲机的中试生产[J].中国生物防治学报,2019,35(5):793-804. |
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| 作者姓名： | 周莲 谢小林 吴天福 陈美标 姚青 朱红惠 |
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| 作者单位： | 1. 广东省微生物研究所, 广东省科学院/华南应用微生物国家重点实验室/广东省菌种保藏与应用重点实验室/广东省微生物菌种保藏中心, 广州 510070;2. 华南农业大学园艺学院, 广州 510642 |
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| 基金项目： | 广东省省级科技计划项目（2017A020224022，2016B090918090）；肇庆市科技计划项目（2017B010136108）；广州市科技计划项目（201906010022）；广东省科学院实施创新驱动发展能力建设专项（2018GDASCX-0909） |
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| 摘 要： | 淡紫拟青霉Paecilomyces lilacinus在线虫生物防治上表现出巨大的潜力，但大规模生产技术的不成熟以及产品货架期短限制其工业化生产。为获得淡紫拟青霉M-1固体发酵的最佳条件，并建立其规模化生产工艺，本研究采用单因素和正交试验对淡紫拟青霉M-1固体发酵培养的组成、发酵条件以及烘干条件进行了优化，结果表明淡紫拟青霉M-1固体发酵最佳的培养基组成为麸皮：玉米粉为1：1、蔗糖添加量5%、尿素添加量0.1%、硫酸铵添加量0.1%、料水比1：0.67，固体发酵最佳的培养条件为培养温度28℃、培养时间为7 d、液体接种量为5%、固体接种量为0.5%，固体菌剂最适烘干条件为在35℃烘干24 h，在此条件下淡紫拟青霉M-1固体菌剂的有效活菌数为9.47×109 CFU/g。在此基础上，基于自动化种曲机，建立淡紫拟青霉M-1规模化固体发酵工艺，并通过3个批次规模化生产进行验证，获得将近2 t淡紫拟青霉M-1固体菌剂产品，菌剂有效活菌数能达到15.6×109 CFU/g，杂菌率极低（<0.01%），水分含量为9.68%。由此说明，该工艺可用于淡紫拟青霉M-1工业化生产，且产品质量优异。
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| 关 键 词： | 淡紫拟青霉M-1 固体发酵 自动化种曲机 分生孢子数 有效活菌数 |
| 收稿时间： | 2019-01-28 |
Optimization of Solid-state Fermentation Process of Paecilomyces lilacinus M-1 and Pilot Production based on Automatic Seed Koji Machine |
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| ZHOU Lian,XIE Xiaolin,WU Tianfu,CHEN Meibiao,YAO Qing,ZHU Honghui.Optimization of Solid-state Fermentation Process of Paecilomyces lilacinus M-1 and Pilot Production based on Automatic Seed Koji Machine[J].Chinese Journal of Biological Control,2019,35(5):793-804. |
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| Authors: | ZHOU Lian XIE Xiaolin WU Tianfu CHEN Meibiao YAO Qing ZHU Honghui |
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| Institution: | 1. Guangdong Institute of Microbiology, Guangdong Academy of Sciences/State Key Laboratory of Applied Microbiology Southern China/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangdong Microbial Culture Collection Center, Guangzhou 510070, China;2. South China Agricultural University, College of Agriculture, Guangzhou 510642, China |
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| Abstract: | Paecilomyces lilacinus has a great potential in biological control of the plant nematodes, however, the immature technology of mass production and short shelf life limit its industrial production. Thus, in order to obtain the optimum conditions for solid fermentation of P. lilacinus M-1 and to establish its industrial production technology, single factor experiment and orthogonal experiment were used in this study to optimize its fermentation conditions, such as culture medium composition, fermentation conditions and drying conditions. The results showed that the optimized composition for solid fermentation media of P. lilacinus M-1 was as followed:bran:corn (1:1), sucrose addition (5%), urea addition (0.1%), ammonium sulfate (0.1%) and the ratio of material to water (1:0.67). The optimum culture conditions of solid fermentation was as followed:culture temperature (28℃), culture time (7 d), liquid inoculation amount (5%) and solid inoculation amount (0.5%). The optimum drying condition of the fungal inoculum was 35℃ for 24 h. Under this optimal condition, the number of living cells in P. lilacinus M-1 solid inoculant was 9.47×109 CFU/g. Then, on this basis, the large-scale solid fermentation process of P. lilacinus M-1 was established by means of automatic seed koji, and verified by 3 batches of larger-scale production, nearly 2 t P. lilacinus M-1 solid inoculant were obtained, which had 15.6×109 CFU/g living cells and had a very low rate of miscellaneous bacteria (<0.01%), and the moisture content was 9.68%. In conclusion, this process can be used in the industrial production of P. lilacinus M-1 and the product quality is excellent. |
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| Keywords: | Paecilomyces lilacinus M-1 solid fermentation automatic seed koji machine spore number living cell |
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