| 对葱黄寡毛跳甲高毒力Bt菌株的鉴定及效果评价 |
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| 引用本文： | 宋健,曹伟平,张晓,杜立新.对葱黄寡毛跳甲高毒力Bt菌株的鉴定及效果评价[J].中国生物防治学报,2019,35(2):197-202. |
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| 作者姓名： | 宋健 曹伟平 张晓 杜立新 |
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| 作者单位： | 1. 河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;2. 河北省农林科学院, 石家庄 050031 |
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| 基金项目： | 国家重点研发计划（2017YFD0201205）；河北省自然科学基金（C2018301023）；河北省财政项目（C19R1001）；河北省农林科学院科学技术研究与发展计划（2018120302） |
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| 摘 要： | 本研究筛选到一株对葱黄寡毛跳甲具有较高杀虫活性的菌株DK49，利用菌株DK49 gyrB基因序列和phoR基因序列分别在GenBank中进行同源性比较，两种基因序列均与苏云金芽胞杆菌的相应基因序列同源性最高，达到99%；同时分别构建系统发育树，发现菌株DK49均与苏云金芽胞杆菌聚合在一起，说明菌株DK49属于苏云金芽胞杆菌。Biolog表型分析结果显示菌株DK49可有效利用14种碳源，对二甲胺四环素等10种化学物质敏感，对利福霉素等11种化学物质不敏感。通过室内生物活性评价，明确菌株DK49对葱黄寡毛跳甲成虫3 d的LC50为0.8161×107 cfu/mL。田间小区结果显示，菌株DK49在1×107 cfu/mL浓度下，连续施药3次后对葱黄寡毛跳甲的虫口减退率达到92.31%，防治效果达到96.81%。可见，菌株DK49能有效地控制葱黄寡毛跳甲的发生为害，具有良好的应用前景。
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| 关 键 词： | 葱黄寡毛跳甲 苏云金芽胞杆菌 分子鉴定 活性评价 |
| 收稿时间： | 2018-10-22 |
Identification and Evaluation of High Virulence Bt Strain against Luperomorpha suturalis Adults |
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| SONG Jian,CAO Weiping,ZHANG Xiao,DU Lixin.Identification and Evaluation of High Virulence Bt Strain against Luperomorpha suturalis Adults[J].Chinese Journal of Biological Control,2019,35(2):197-202. |
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| Authors: | SONG Jian CAO Weiping ZHANG Xiao DU Lixin |
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| Institution: | 1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;2. Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050031, China |
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| Abstract: | A strain DK49 of high insecticidal activity against Luperomorpha suturalis was screened. Homology of the strain DK49 was compared using gene sequences of gyrB and phoR in GenBank separately, which showed high homology (99%) to that of Bacillus thuringiensis. Phylogenetic trees indicated that the strain DK49 is a B. thuringiensis strain. Biolog phenotypic analysis showed that the strain DK49 could effectively absorb 14 carbon sources and was sensitive to 10 chemicals and insensitive to 11 chemicals. Laboratory evaluation of bioactivity determined its 3 d LC50 against L. suturalis adult to be 0.8161×107 cfu/mL. Field assays with 3 continuous sprays at 1×107 cfu/mL showed a control efficiency of 96.81% against L. suturalis. Therefore the strain DK49 has a potential for field control of L. suturalis. |
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| Keywords: | Luperomorpha suturalis Bacillus thuringiensis molecular identification activity evaluation |
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