Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice |
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| Authors: | Satoshi Watanabe Kazuo Kumakura Hideki Kato Hiroyuki Iyozumi Masayuki Togawa Kozo Nagayama |
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| Institution: | (1) Kumiai Chemical Industry, Life Science Research Institute, Kikugawa, Shizuoka 439-0031, Japan;(2) Research and Development Department, Kumiai Chemical Industry, Tokyo, Japan;(3) Research and Development Division, K · I Chemical Industry, Shizuoka, Japan;(4) Shizuoka Agricultural Experiment Station, Shizuoka, Japan |
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| Abstract: | Trichoderma SKT-1 was previously reported as a powerful biological control agent against seedborne pathogens of rice, but the taxonomic disposition of the fungal isolate was not clear. Trichoderma SKT-1 produced irregular pyramidal warts on conidia and had an optimum growth temperature of 30°C. Morphological characteristics and colony growth were identical to those of known species of Trichoderma, including the newly recognized species T. asperellum. The 5.8S rDNA with the internal transcribed spacer (ITS) region (ca. 514 bp) of the fungus was compared with those of known species to determine the phylogenetic placement of the fungus. The length and sequence of the regions from Trichoderma SKT-1 were completely identical to those of an isolate of T. asperellum NRRL 5242 (AJ230669). On the basis of these results, we concluded that Trichoderma SKT-1 was T. asperellum. |
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| Keywords: | Biological control Trichoderma SKT-1 T asperellum Conidial surface ITS phylogeny |
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