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| 盐酸环丙沙星在患子宫内膜炎奶牛子宫内给药的药动学研究 | |
| 引用本文： | 陶金忠,杨国林,李剑勇,李世宏,赵兴绪,严作庭,谢家声,巩忠福.盐酸环丙沙星在患子宫内膜炎奶牛子宫内给药的药动学研究[J].中国奶牛,2008(2):7-10. |
| 作者姓名： | 陶金忠 杨国林 李剑勇 李世宏 赵兴绪 严作庭 谢家声 巩忠福 |
| 作者单位： | 1. 宁夏大学农学院,银川,750021;甘肃农业大学,兰州,730070;中国农业科学院兰州畜牧与兽药研究所,兰州,730050 2. 中国农业科学院兰州畜牧与兽药研究所,兰州,730050 3. 甘肃农业大学,兰州,730070 |
| 摘 要： | 本研究以反相高效液相色谱为定量分析手段,采用5头患子宫内膜炎的奶牛,通过子宫内灌注盐酸环丙沙星（2.5g/头）,研究了盐酸环丙沙星在患子宫内膜炎奶牛体内的药代动力学规律。以二氟沙星为内标,血浆样品经甲醇沉淀蛋白,离心,经针头式过滤器处理,用反相高效液相法测定其中盐酸环丙沙星的浓度。色谱条件为：ODS-1C18柱;测定流动相为0.015mol/L四丁基溴化铵溶液-乙腈（92∶8,V/V）,pH为3.0;流速为1.0mL/min;荧光检测器,激发波长（λex）278nm,发射波长（λem）465nm。通过采用MCPKP房室分析程序,分析血中浓度-时间数据,发现有三头牛血样药时数据符合无吸收三室开放模型。其血样中主要药动学参数为：T1/2α为0.916h、T1/2β为49.20h、AUC高达7.6296mg/（L·h）、Clβ为1.582L/（kg·h）。有两头牛血样药时数据符合一级吸收二室开放模型。其主要药动学参数为：T1/2α为1.26h、T1/2β为10h、AUC高达28.336mg/（L·h）。试验结果表明,盐酸环丙沙星子宫给药吸收快,分布广泛,消除慢。 |
| 关 键 词： | 盐酸环丙沙星 药动学 反相高效液相色谱法 子宫内膜炎 奶牛 |
| 文章编号： | 1004-4264（2008）02-0007-03 |
| 收稿时间： | 2007-07-11 |
| 修稿时间： | 2007年7月11日 |
Study of Pharmacokinetics of Ciprofloxacin Hydrochloride in the Uterus of Endometritis Cows |
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| Tao Jinzhong,Yang Guolin,Li Jianyong,Li Shihong,Zhao Xingxu,Yan Zuoting,Xie Jiasheng,Gong Zhongfu.Study of Pharmacokinetics of Ciprofloxacin Hydrochloride in the Uterus of Endometritis Cows[J].China Dairy Cattle,2008(2):7-10. | |
| Authors: | Tao Jinzhong Yang Guolin Li Jianyong Li Shihong Zhao Xingxu Yan Zuoting Xie Jiasheng Gong Zhongfu |
| Institution: | Tao Jinzhong, Yang Guolin, Li Jianyong, Li Shihong, Zhao Xingxu, Yah Zuoting , Xie Jiasheng , Gong Zhongfu(1.School of Agriculture of Ningxia University ,Yinchuan 750021; 2.Faculty of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070;3.Lanzhou Institute of Pasturage and Veterinary Medicine of Chinese Academy of Agricultural Sciences, Lanzhou 730050) |
| Abstract: | The pharmacokinefics and residues of ciprofloxacin hydrochlofide in the milk were investigated in 5 cows suffered from endometrifis following single-intrauterine administration at the dosage of 2.5g per cow. Blood and milk were sampled at different intervals after administration and ciprofloxacin hydrochlofide concentrations were determined by RP-HPLC method with a fluorescent detector. Difloxacin was used as intra-standard. The data were analyzed with MCPKP program. The ciprofloxacin hydrochlofide concentration-time data were fitted to a two-compartment open model with first order absorption in two cows suffered from endometritis after single-intrauterus. The main pharmacokinetics parameters were as follow：T1/2α 1.26h, T1/2β10h, AUC 28.336mg/（L. h）, 13 0. 3571 h^-1. The ciprofloxacin hydrochloride concentration-time data were fitted to a three-compartment open model with non-absorption in other three cows. The main pharmacokinetics parameters were as follow：T1/2α .916h, T1/2β49.20h, AUC 7.6296mg/（L·h）, Clβ 1.582 l/（kg·h）, 13 0.642h^-1. The results showed that ciprofloxaxin hydrochloride was absorbed fast and distributed extensively, but it was eliminated slowly in the blood after single-intrauterine administration. |
| Keywords: | Ciprofloxacin hydrochloride Pharmacokinetics RP-HPLC Endometritis Cow |
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