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| H-ATPase弱化菌株的筛选及其应激性研究 | |
| 引用本文： | 霍贵成,崔兰,刘飞.H-ATPase弱化菌株的筛选及其应激性研究[J].东北农业大学学报,2012(5):1-6. |
| 作者姓名： | 霍贵成 崔兰 刘飞 |
| 作者单位： | 1. 乳品科学教育部重点实验室,哈尔滨150030 2. 东北农业大学食品学院,哈尔滨150030 3. 丹尼斯克中国有限公司,江苏昆山215300 |
| 基金项目： | 国家自然科学基金(30972131);东北农业大学博士启动基金(2009RC31) |
| 摘 要： | 利用新霉素作为筛选压力,筛选H+-ATPase缺陷的德氏乳杆菌保加利亚亚种自发突变株,筛选出一株突变菌株,命名为 KLDS1.9201-11.比较亲本菌株 KLDS1.9201与突变菌株 KLDS1.9201-11的生长情况,发现KLDS1.9201-11的生长活力较亲本菌株差且产酸能力弱;在pH 3.0的MRS液体培养基中经过3 h酸应激后,亲本菌株和突变菌株的活菌数分别降低86.98%和99.98%,酶活分别降低13.33%和21.15%;在冻干后,亲本菌株和突变菌株的活菌数分别降低76.71%和4.88%,亲本菌株的酶活降低4.27%,而突变菌株的酶活上升29.71%.结果表明,突变菌株较亲本菌株具有更强的酸敏感性和冷适应性,可以用于弱后酸化酸奶发酵剂的制备 |
| 关 键 词： | 德氏乳杆菌保加利亚亚种 H+-ATPase 酸应激 冷应激 |
Derivation of H~+-ATPase-defective mutant from Lactobacillus delbru-eckii subsp.bulgaricus KLDS1.9201 and its stress response |
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| HUO Guicheng,CUI Lan,LIU Fei.Derivation of H~+-ATPase-defective mutant from Lactobacillus delbru-eckii subsp.bulgaricus KLDS1.9201 and its stress response[J].Journal of Northeast Agricultural University,2012(5):1-6. | |
| Authors: | HUO Guicheng CUI Lan LIU Fei |
| Institution: | 1,2,3(1.Key laboratory of Dairy Science,Ministry of Education,Harbin 150030,China;School of Food Science,Northeast Agricultural University,Harbin 150030,China,3.Danisco(China) Co.,Ltd.,Kunshan Jiangsu 215300,China) |
| Abstract: | One spontaneous neomycin mutant strain named KLDS1.9201-11 was obtained from Lactobacillus delbrueckii subsp.bulgaricus KLDS1.9201,which showed the reduced H+-ATPase activity.Compared with parent strain,the mutant strain had lower growth rate and acid yield ability.The acid tolerance of the two strains were compared by using three-hour-exposure process to acidic conditions with pH 3.0,results showed that the viable count of the parent strain and mutant strain reduced by 86.98% and 99.98%,separately,and the membrane-bound H+-ATPase activities of the parent strain and mutant strain reduced by 13.33% and 21.15%,respectively.The freeze-drying test showed that the viable count of the parent strain and mutant strain reduced by 76.71% and 4.88%,respectively,and the membrane-bound H+-ATPase activities of the parent strain reduced by 4.27%,while which of the mutant strain increased by 29.71%.In conclusion,compared to the parent strain,the mutant strain had higher sensitivity to acid and cold tolerance ability,which could be used to make the starter culture with low-postacidification ability. |
| Keywords: | Lactobacillus delbrueckii subsp bulgaricus H+-ATPase acid stress cold stress |
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