| 蜡样芽孢杆菌特征性蛋白的分离与纯化 |
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| 引用本文： | 吴海清,陈庆森.蜡样芽孢杆菌特征性蛋白的分离与纯化[J].天津农学院学报,2011,18(2):20-22. |
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| 作者姓名： | 吴海清 陈庆森 |
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| 作者单位： | 1. 天津农学院食品科学系,天津,300384
2. 天津商业大学生物技术与食品科学学院,天津,300134 |
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| 基金项目： | 国家科技攻关计划重大专题项目"乳品质量安全监测关键技术研究与应用" |
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| 摘 要： | 蜡样芽孢杆菌是一种致病性病原菌,能引起胃肠感染和非胃肠感染。本研究从实验室分离的蜡样芽孢杆菌、蜡样芽孢杆菌1.026 0、巨大芽孢杆菌1.015 1、蕈状芽孢杆菌1.085 6和苏云金芽孢杆菌1.101 4这五株菌中提取蛋白,利用SDS-PAGE方法纯化,并测定出蜡样芽孢杆菌有3种分子量约为28.5、26.5和20.0 KDa的特征性蛋白,而苏云金芽孢杆菌、巨大芽孢杆菌和蕈状芽孢杆菌则没有此特征性蛋白。
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| 关 键 词： | 蜡样芽孢杆菌 特征性蛋白 SDS-PAGE |
Isolation and Purification of Characteristic Proteins of Bacillus cereus |
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| WU Hai-qing,CHEN Qing-sen.Isolation and Purification of Characteristic Proteins of Bacillus cereus[J].Journal of Tianjin Agricultural College,2011,18(2):20-22. |
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| Authors: | WU Hai-qing CHEN Qing-sen |
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| Institution: | wu Hai - qing, CHEN Qing - sen ( 1. Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China; 2. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China) |
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| Abstract: | Bacillus cereus is a causative agent in both gastrointestinal and in nongastrointestinal infections. The proteins were separated from Bacillus cereus, Bacillus cereus 1. 026 0, B. thuringiensis 1. 015 1, B. megaterium 1. 085 6 and B. mycoides 1. 101 4, then they were purified by SDS - PAGE. Finally, the three kinds of characteristic protein were got in B. cereus, and the molecular weights were approximately 28.5, 26.5 和 20 KDa, while the B. thuringiensis, B. megaterium and B. mycoides didn t have the three kinds of characteristic protein. |
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| Keywords: | Bacillus cereus characteristic protein SDS - PAGE |
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