| 鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析 |
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| 引用本文： | 黄海东,詹欣娜,李娜.鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析[J].天津农学院学报,2013(3):1-4. |
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| 作者姓名： | 黄海东 詹欣娜 李娜 |
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| 作者单位： | 天津农学院农学系,天津,300384 |
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| 基金项目： | 天津市自然科学基金重点项目“新型鞘氨醇胶合成关键基因的研究” |
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| 摘 要： | 以自杀性质粒pUT携带的mini—Tn5转座子对菌株TP-3进行转座诱变，从转座突变库中筛选到不能合成产物鞘氨醇胶ss的突变株，进而采用Tn5外向扩增与LP—RAPD／fN结合的方法，得到糖基转移酶编码基因spsK的801bp序列全长。生物信息学分析表明，其氨基酸序列与迪特胶以及S88合成途径中的糖基转移酶同源性最高，达47％；SpsK蛋白具有典型的G—loop和DXD保守模式，属于糖基转移酶的GT—A家族。
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| 关 键 词： | 鞘氨醇单胞菌 鞘氨醇胶Ss 糖基转移酶 生物信息学 |
Cloning and Bioinformatics Analysis ofspsK Gene fromSphingomonas sanxanigenens sp. nov. TP-3 |
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| HUANG Hai-dong
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ZHAN Xin-na
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LI Na.Cloning and Bioinformatics Analysis ofspsK Gene fromSphingomonas sanxanigenens sp. nov. TP-3[J].Journal of Tianjin Agricultural College,2013(3):1-4. |
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| Authors: | HUANG Hai-dong ZHAN Xin-na LI Na |
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| Institution: | (Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China) |
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| Abstract: | Strian TP-3 was induced by transposon mini-Tn5 on suicide plasmid pUT, and a mutant strain which could not produce sphingan Ss was screened. The spsK gene of TP-3 was obtained by the method of Tn5 flanking PCR and LP-RAPD. The spsK gene coding region includes 801 bp and encodes glycosyl transferase. The bioinformatics analysis reveals that spsK from strain TP-3 showed the highest of 47% amino acid sequence identity with glycosyl transferase from biosynthesis of diutan and S88 sphingan. SpsK protein from strain TP-3 belongs to the glycosyl transferase GT-A family with the typical motif of G-loop and DXD. |
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| Keywords: | Sphingomonassp sphingan Ss glycosyl transferase bioinformatics |
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