| 火龙果茎腐病拮抗细菌的筛选和鉴定 |
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| 引用本文： | 逄崇洋,张荣意,谭志琼.火龙果茎腐病拮抗细菌的筛选和鉴定[J].中国果业信息,2017,46(3). |
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| 作者姓名： | 逄崇洋 张荣意 谭志琼 |
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| 作者单位： | 海南省热带生物资源可持续利用重点实验室,海南省热带生物资源可持续利用重点实验室,海南省海口市海南大学热带农林学院 |
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| 基金项目： | 基于诊断技术的热区植物病理学课程资源库建设（项目编号Hnjg2016ZD-2/hdjy1606）. |
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| 摘 要： | 摘要：寻找1-2株对火龙果茎腐病具有拮抗作用的细菌菌株。通过对黑龙江、江苏、浙江、海南等地的土壤中的细菌进行分离纯化，得到细菌菌株206株。采用平板对峙法筛选出对火龙果茎腐病菌具有强烈拮抗作用的菌株1株，PCY－67。菌株能够显著抑制火龙果茎腐病菌菌丝生长，抑菌圈直径分别可达30mm。通过形态特征、生理生化特性及16S rDNA序列同源性比较，鉴定该菌株均为类芽孢杆菌（Paenibacillus polymyxa Ash, Priest & Collin）。
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| 关 键 词： | 关键词：火龙果茎腐病 拮抗菌 筛选鉴定 |
| 收稿时间： | 2017/2/27 0:00:00 |
| 修稿时间： | 2017/3/7 0:00:00 |
Screening and identification of antagonistic bacteria against Hylocereus neoscytalidium stem rot |
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| zhangrongyi and tanzhiqiong.Screening and identification of antagonistic bacteria against Hylocereus neoscytalidium stem rot[J].China Fruit News,2017,46(3). |
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| Authors: | zhangrongyi and tanzhiqiong |
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| Abstract: | Abstract: Find 1 to 2 bacterial strain with strong antagonistic effect on Hylocereus Neoscytalidium Stem rot.By means of the separation and purification of bacteria from the soil in Heilongjiang, Jiangsu, Zhejiang, Hainan and other places, 206 bacterial strains were obtained. The plate confrontation method was used to screen a bacterial strain with strong antagonistic effect on Hylocereus Neoscytalidium Stem rot, which is the PCY 67. This bacterial strain can significantly inhibit the growth of Hylocereus Neoscytalidium Stem rot, the diameter of inhibition zone can up to 30mm.Through the comparison of physiology, biochemistry, and morphological characteristics, as well as the 16S rDNA sequence homology, the strain was identified as Paenibacillus polymyxa Ash, Priest & Collin. |
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| Keywords: | Keywords:Hylocereus neoscytalidium stem rot antagonistic bacteria isolation and identification |
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