Needle blight caused by Dothistroma pini in Slovakia: distribution,host range and mating types |
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| Authors: | Emília Ondru?ková Zuzana He?ková-Jáno?íková Slavomír Adam?ík Miriam Kádasi Horáková Dominika Rakúsová-Sládková Katarína Adam?íková |
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| Institution: | 1. Department of Plant Pathology and Mycology, Institute of Forest Ecology Slovak Academy of Sciences, Nitra, Slovak Republic;2. Plant Science and Biodiversity Center Slovak Academy of Science, Institute of Botany, Bratislava 4, Slovak Republic;3. Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic |
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| Abstract: | Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia. |
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| Keywords: | Dothistroma needle blight pine species phylogenetic analysis ITS EF1-α ß-tubulin 2 regions |
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