| 免疫亲和柱—高效液相色谱法检测乳制品中的磺胺 |
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| 引用本文： | 谢洁,张清杰,果旗,江帆,胡明勋,王雄,邓健.免疫亲和柱—高效液相色谱法检测乳制品中的磺胺[J].农产品加工．学刊,2012(10):23-25. |
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| 作者姓名： | 谢洁 张清杰 果旗 江帆 胡明勋 王雄 邓健 |
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| 作者单位： | 南华大学化学化工学院;北京中检维康技术有限公司 |
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| 基金项目： | 科技部科技型中小企业技术创新基金(11C26211103846);质检公益性行业科研专项(201210086) |
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| 摘 要： | 建立了牛奶和奶粉中16种磺胺残留物的高效液相色谱—紫外快速测定方法。样品用乙酸乙酯提取,所得清液经免疫亲和柱净化富集,流动相为甲醇和1.1%乙酸—0.01 mol PBS溶液(梯度洗脱),应用液相色谱—紫外法检测。该方法的回收率为80%～110%,相对标准偏差小于10%;方法检测牛奶样品的灵敏度为0.5μg/L,奶粉样品的灵敏度为5.0μg/kg。
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| 关 键 词： | 磺胺残留物检测 免疫亲和柱净化富集 液相色谱—紫外法 牛奶 |
Rapid Method for the Determination of Sulfonamides in Milk and Powder Milk by Immunoaffinity Column Clean-up and HPLC-UV |
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| XIEJie,ZHANG Qing-jie,GUO Qi,JIANG Fan,HU Ming-xun,WANG Xiong,DENGJian.Rapid Method for the Determination of Sulfonamides in Milk and Powder Milk by Immunoaffinity Column Clean-up and HPLC-UV[J].Nongchanpin Jlagong.Xuekan,2012(10):23-25. |
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| Authors: | XIEJie ZHANG Qing-jie GUO Qi JIANG Fan HU Ming-xun WANG Xiong DENGJian |
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| Institution: | 1 (1.College of Chemistry and Chemical Engineering University of South China,Hengyang,Hu’nan 421001,China; 2.Clover Technology Group Limited,Beijing 100044,China) |
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| Abstract: | A high performance liquid chromatography-UV method is established for the determination of 16 sulfonamides residues in milk and powder milk clean up by immunoaffinity column(IAC).After extraction with ethyl acetate,the clear part is loaded on the IAC column for purification,the mobile phase is methanol-1.1%acetic acid-0.01 mol PBS with gradient elution,and the chromatographic analysis is performed by HPLC.The recoveries of the method are in the range of 80%~ 110%,the RSD is below 10%,the limit of quantification for milk and powder milk is 0.5μg/L and 5.0μg/kg,respectively. |
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| Keywords: | sulfonamides immunoaffinity column HPLC milk |
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