| 羽衣甘蓝叶黄素CO_2超临界萃取及高速逆流色谱纯化的研究 |
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| 引用本文： | 王向东,于有伟,段平肖,张秀香.羽衣甘蓝叶黄素CO_2超临界萃取及高速逆流色谱纯化的研究[J].农产品加工．学刊,2012(10):14-15,20. |
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| 作者姓名： | 王向东 于有伟 段平肖 张秀香 |
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| 作者单位： | 山西师范大学 |
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| 基金项目： | 山西省自然科学基金研究项目(2007011099) |
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| 摘 要： | 以羽衣甘蓝冻干粉末为原料,利用超临界CO_2流体技术萃取羽衣甘蓝叶黄素粗品;选择中极性、弱极性、极弱极性的溶剂体系,进行粗品的高速逆流色谱纯化。结果表明,萃取温度35℃,萃取压力25 MPa,主泵频率30 Hz,叶黄素粗品萃取量最高,达到32.43 mg/g;采用正己烷—乙醇—水(6:5:1.5)溶剂体系,纯化效果最好,得到纯度98.5%的羽衣甘蓝叶黄素。
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| 关 键 词： | 羽衣甘蓝 叶黄素 超临界CO_2 萃取 高速逆流色谱 纯化 |
Supercritical CO2 Extraction of Lutein from Kale and Its High-speed Counter-current Chromatography Purification |
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| WANG Xiang-dong,YU You-wei,DUAN Ping-xiao,ZHANG Xiu-xiang.Supercritical CO2 Extraction of Lutein from Kale and Its High-speed Counter-current Chromatography Purification[J].Nongchanpin Jlagong.Xuekan,2012(10):14-15,20. |
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| Authors: | WANG Xiang-dong YU You-wei DUAN Ping-xiao ZHANG Xiu-xiang |
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| Institution: | 1.Shanxi Normal University,Lin Fen,Shanxi 041004,China; 2.TunLi Town,Linfen City,Lin Fen,Shanxi 041000,China) |
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| Abstract: | Taking kale as raw material,after smashing,cooling and drying,lutein is supposed to extract from kale powder by supercritical carbon dioxide.After the extraction,three solvent besides neutral,weak and the weaker solvent are chosen to purify the extracted lutein by high -speed counter -current chromatography.The result shows that in the condition of extraction temperature being 35℃,extraction pressure being 25 MPa,and the main pump frequency being 30 Hz,the quantity of lutein extraction is the highest,32.43 mg/g.It is also got that the solvent systems of hexane-ethanol-water(6:5: 1.5) purification effect is the best,and purity of kale lutein is 98.5%. |
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| Keywords: | kale lutein supercritical CO_2 extraction high-speed counter-current chromatography purification |
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