| 紫外分光光度法测定罗汉果中黄酮类化合物 |
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| 引用本文： | 荣德福,赵晔萍.紫外分光光度法测定罗汉果中黄酮类化合物[J].农产品加工．学刊,2012(10):128-130. |
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| 作者姓名： | 荣德福 赵晔萍 |
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| 作者单位： | 北仑出入境检验检疫局;北仑区环境保护局 |
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| 摘 要： | 选用紫外分光光度法测定了罗汉果中黄酮类化合物的含量,并得到了满意结果。同时,研究了甲醇的水溶液作溶剂回流提取罗汉果中黄酮类化合物,分别对甲醇浓度、浸泡时间及投料率等条件进行了最优化试验,当甲醇体积分数50%,浸泡时间1.5 h,投料率为1:10时,黄酮提取率最佳。用芦丁为基准物,在波长362 nm处用紫外分光光度法分析其含量,相关系数r>0.99,测得甲醇水溶液提取罗汉果中黄酮类化合物含量为1%左右,本方法可以用于实际测定罗汉果中黄酮类化合物含量。
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| 关 键 词： | 罗汉果 甲醇水溶液 黄酮类化合物 紫外分光光度法 |
The Content Test of Flavonoids in Siraitia Grosvenorii by UV |
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| RONGDe-fu,ZHAO Ye-ping.The Content Test of Flavonoids in Siraitia Grosvenorii by UV[J].Nongchanpin Jlagong.Xuekan,2012(10):128-130. |
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| Authors: | RONGDe-fu ZHAO Ye-ping |
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| Institution: | 1.Beilun Entry-exit Inspection and Quarantine Bureau,Ningbo,Zhejiang 315800,China; 2.Beilun Environment Protection Bureau,Ningbo,Zhejiang 315800,China) |
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| Abstract: | This article tests the content of flavonoids in Siraitia grosvenorii by UV and the result is satisfied.The Methanol-H_2O dissolvent backflow to extract flavonoids in Siraitia grosvenorii is researched,and the content of MeOH-H_2O,the time of soaking and the mass ratio of the Siraitia grosvenorii and the dissolvent are optimized.It is found that optimal conditions is 50%,1.5 h,1:10.The rutin as reference material,the content of flavonoids is test using UV in 362 nm,r>0.99.The content of flavonoids in Siraitia grosvenorii is about 1%.The method can be used to determine the content of flavonoids in Siraitia grosvenorii. |
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| Keywords: | Siraitia grosvenorii MeOH-H_2O flavonoids UV |
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