| 解淀粉芽孢杆菌产抗菌物质发酵工艺研究 |
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| 引用本文： | 魏红,刘素花,宋庆丰,包放,尚巧霞,赵晓燕,魏艳敏.解淀粉芽孢杆菌产抗菌物质发酵工艺研究[J].中国农学通报,2009,25(11):151-155. |
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| 作者姓名： | 魏红 刘素花 宋庆丰 包放 尚巧霞 赵晓燕 魏艳敏 |
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| 作者单位： | 北京农学院植物科学技术系,北京,102206 |
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| 基金项目： | 北京市教委资助项目，，，北京市大学生科学研究与创业行动计划 |
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| 摘 要： | 摘要：解淀粉芽孢杆菌 （Bacillus amyloliquefaciens）BJ-6 发酵过程中可产生对多种植物病原真菌有抑菌作用的抗菌物质，为提高该菌株抗菌物质的产量，本研究收集到应用于芽孢杆菌发酵的培养基配方18种，通过摇床培养筛选出较适宜于解淀粉芽孢杆菌BJ-6发酵的培养基7种。在此基础上，利用2号培养基对该菌株进行了5L发酵罐的发酵培养，并采用不同生化检测方法测定了发酵过程中菌体数量、蛋白产生量、碳源和氮源的消耗量随发酵时间的动态变化情况，明确了最佳发酵时间为96h以及碳氮源的补加时间为开始发酵后的8h-12h。同时，发酵罐自动记录了发酵过程中pH值和溶氧的动态变化。
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| 关 键 词： | 芽孢杆菌 抗菌物质 发酵 |
| 收稿时间： | 2008-12-26 |
| 修稿时间： | 2009-01-29 |
Studies on Fermentation Process of Bacillus amyloliquefaciens BJ-6 |
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| Wei Hong,Liu Suhu,Song Qingfeng,Bao Fang,Shang Qiaoxi,Liu Zhengping,Wei Yanmin.Studies on Fermentation Process of Bacillus amyloliquefaciens BJ-6[J].Chinese Agricultural Science Bulletin,2009,25(11):151-155. |
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| Authors: | Wei Hong Liu Suhu Song Qingfeng Bao Fang Shang Qiaoxi Liu Zhengping Wei Yanmin |
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| Institution: | (Department of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206) |
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| Abstract: | Abstract: The strain Bacillus amyloliquefaciens BJ-6 can secrete antagonistic substances during the fermentation process, which can inhibit many different kinds of plant pathogenic fungi. In order to improve the yield of antagonistic substances, 18 different kinds of fermentation medium for Bacillus sp. were tested and 7 medium which suitable to ferment the Bacillus amyloliquefaciens BJ-6 have been selected out by shaking culture. On this basis, the strain was cultured by No. 2 medium in 5L fermentor. Biochemical methods were used to detect alive bacteria count, protein produced amounts, consumption amounts of carbon and nitrogen source. It showed that the best fermentation time is 96h and the best supplementary time of carbon and nitrogen source is 8h-12h. During the fermentation process, pH and dissolved oxygen concentration have been recorded automatically by fermentor. |
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| Keywords: | Bacillus antifungal substance fermentation |
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