| 盐度对牟氏角毛藻生长及总脂含量的影响 |
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| 引用本文： | 班剑娇,雷静静,王志强,张旭芳,冯佳,谢树莲.盐度对牟氏角毛藻生长及总脂含量的影响[J].安徽农业科学,2013,41(6):2621-2622. |
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| 作者姓名： | 班剑娇 雷静静 王志强 张旭芳 冯佳 谢树莲 |
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| 作者单位： | 山西大学生命科学学院,山西太原,030006 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的]研究不同盐度对牟氏角毛藻(Chaetoceros muelleri)生长及总脂含量的影响,以确定最高油脂产率的盐度。方法]采用微挑法进行藻株的分离纯化;采用血球计数法进行细胞计数、干重法测定藻株生物量;采用氯仿-甲醇法进行藻株总脂含量的测定。结果]盐度为20‰时,牟氏角毛藻生长最快,最大细胞密度为2.1×106个/ml,干重为58.89 mg/L,;盐度为25‰时,牟氏角毛藻总脂含量最高,达到29.93%。结论]综合评价其生长及总脂积累,得出盐度为20‰时,牟氏角毛藻总脂产率最高,但与盐度25‰时差异不大。
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| 关 键 词： | 牟氏角毛藻 盐度 生物量 总脂含量 |
Effects of Varying Salinity on Growth and Total Lipid Content of Chaetoceros muelleri |
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| Institution: | BAN Jian-jiao et al(School of Life Science,Shanxi University,Taiyuan,Shanxi 030006) |
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| Abstract: | Objective] The effects of three different salinity on growth and total lipid content of Chaetoceros muelleri were researched to obtain the salinity under the highest yield of oil.Method] C.muelleri was isolated and purified by micro-picking method;blood count method was used for determining cell density and dry weight method was used for determining biomass;total lipid content was measured by chloroform-methanol extraction method.Result] The results showed that the highest cell density（2.1×106 ind/ml） and dry weight（58.89 mg/L） of C.muelleri were obtained at salinity of 20‰.The highest total lipid content（29.93%） was obtained at salinity of 25‰.Conclusion] Under salinity of 20‰,the highest lipid productivity was obtained.There is no significant difference between salinity of 20‰ and 25‰. |
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| Keywords: | Chaetoceros muelleri Salinity Biomass Total lipid content |
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