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| 马蔺不同生长阶段嫩叶POD和EST同工酶分析 | |
| 引用本文： | 李强栋,孟林,毛培春,张德罡,韩润英.马蔺不同生长阶段嫩叶POD和EST同工酶分析[J].草原与草坪,2011,31(6):7-13. |
| 作者姓名： | 李强栋 孟林 毛培春 张德罡 韩润英 |
| 作者单位： | 1. 甘肃农业大学草业学院／草业生态系统教育部重点实验室／甘肃省草业工程实验室／中-美草地畜牧业可持续发展研究中心,甘肃兰州730070／北京市农林科学院北京草业与环境研究发展中心,北京100097 2. 北京市农林科学院北京草业与环境研究发展中心,北京,100097 3. 甘肃农业大学草业学院／草业生态系统教育部重点实验室／甘肃省草业工程实验室／中-美草地畜牧业可持续发展研究中心,甘肃兰州,730070 4. 内蒙古通辽市畜牧兽医科学研究所,内蒙古通辽,028000 |
| 基金项目： | 北京市自然科学基金项目 |
| 摘 要： | 利用聚丙烯酰胺凝胶电泳技术，检测了11份不同居群马蔺种质材料叶片过氧化物酶（POD）、酯酶（EST）同工酶的2个酶系统22个同工酶位点，分析马蔺营养生长和生殖生长阶段的同工酶变化。结果表明，11份马蔺种质叶片共有13种POD酶带，9种EST酶带，其中，基本谱带8条，特征谱带4条；与生殖生长阶段相比较，马蔺营养生长阶段E... |
| 关 键 词： | 马蔺 营养生长 生殖生长 POD EST |
Isozyme analysis of leaf buds in different growth stages of 11 Chinese Iris populations |
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| LI Qiang-dong,MENG Lin,MAO Pei-chun,ZHANG De-gang,HAN Run-ying.Isozyme analysis of leaf buds in different growth stages of 11 Chinese Iris populations[J].Grassland and Turf,2011,31(6):7-13. | |
| Authors: | LI Qiang-dong MENG Lin MAO Pei-chun ZHANG De-gang HAN Run-ying |
| Institution: | 1. College of Pratacultural Science ,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education / Pratacultural Engineering Laboratory of Gansu Province / Sino-U. S. Centers for Grazingland Ecosystem Sustainability Lanzhou 730070,China ; 2. Beijing Research and Development Center for Grasses and Environment,Beijing Academy of Agriculture and Forestry Sciences ,Beijing 100097,China; 3. Tongliao Institute of Veterinary and Animal Sciences, Tongliao 028000, China) |
| Abstract: | The POD and EST isozyme banding patterns were studied for wild germplasm and materials of Chinese Iris through vertical polyacrylamide gel electrophoresis. Different activity levels and phenotypic groups were detected in studied enzyme systems. In tested 11 germplasms and materials,21 POD and EST loci were obtained from 2 enzyme systems of 11 Chinese Iris in vegetative and reproductive growth stages. The result showed that the 22 enzyme bands presented at the tested materials,13 of which were presented at POD zymo- gram and nine at EST zymogram,8 basic enzyme bands and 4 specific enzyme bands were respectively obtained. There were more isozyme bands obtained during vegetative stage and POD bands in reproductive stage were clearer than that in vegetative stage,EST bands were clear during vegetative. In addition, the leaf bud in vegetative growth stage was the best for EST test,while the one in reproductive growth stage was the best for POD test. |
| Keywords: | Iris lactea Pall var chinensis vegetative growth stage reproductive growth stage POD EST |
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