| 日本囊对虾性腺蛋白质双向电泳的样品制备方法的改进 |
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| 引用本文： | 岳亮,王艺磊,张子平,冯建军,邹志华,谢芳靖,林鹏.日本囊对虾性腺蛋白质双向电泳的样品制备方法的改进[J].厦门水产学院学报,2011(4):246-251. |
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| 作者姓名： | 岳亮 王艺磊 张子平 冯建军 邹志华 谢芳靖 林鹏 |
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| 作者单位： | [1]集美大学水产学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021 [3]德克萨斯州立大学化学和生物化学系,德克萨斯圣马克斯美国TX78666 |
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| 基金项目： | 国家自然科学基金资助项目（30972281）;福建省高等学校新世纪优秀人才支持计划项目（闽教科[2007]20号）;集美大学创新团队基金资助项目（2010A001） |
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| 摘 要： | 采用一种能同时提取RNA、DNA和Protein的试剂（RDP试剂）进行日本囊对虾性腺总蛋白质的制备，并通过双向电泳（2-DE）技术与传统的裂解液法比较提取效果．RDP法所得2-DE图谱清晰，条纹少，蛋白质点数多，而裂解液法存在横向和纵向拖尾，其碱性端蛋白质点大量缺失，说明RDP法能有效去除蛋白质样品中盐离子、脂类等干扰成分．结果不仅为后续的日本囊对虾性腺蛋白质组学研究提供了技术保障，也为其他甲壳类动物性腺总蛋白质的提取提供了借鉴．
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| 关 键 词： | RDP法 双向电冰 日本囊对虾 裂解液法 |
A Novel Preparation Method of Marsupenaeus japonicus Gonad Samples for Two-dimensional Electrophoresis |
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| Authors: | YUE Liang WANG Yi-lei ZHANG Zi-ping FENG Jian-jun ZOU Zhi-hua XIE Fang-jing LIN Peng |
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| Institution: | 1. Fisheries College, Jimei University, Xiamen 361021, China; 2. The Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University), Fujian Province, Xiamen 361021, China; 3. Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA) |
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| Abstract: | The gonad total protein of penaeid shrimp Marsupenaeus japonicus was prepared by a special reagent used to extract DNA, RNA and protein simultaneously （RDP reagent）. The extraction efficiency was compared with traditional lysis solution method by two-dimensional eleetrophoresis （2-DE）. The 2-DE map of RDP was clear with less striations and more protein spots, while that of lysis solution method had horizontal and vertical striations with decrease of protein spots at the cathodic side. These results indicated that RDP method could remove salt and lipid from gonad sample effectively. The finding from the study provided not on- ly technical support for proteomies research of M. japonicus , but also useful reference for gonad total protein extraction of other crustacean. |
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| Keywords: | RDP method two-dimensional electrophoresis Marsupenaeus japonicus lysis solution method |
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