| 犬瘟热病毒YD株的分离鉴定及其H基因序列分析 |
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| 引用本文： | 张建普,马丽利,周利锋.犬瘟热病毒YD株的分离鉴定及其H基因序列分析[J].畜牧与兽医,2021(4):69-75. |
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| 作者姓名： | 张建普 马丽利 周利锋 |
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| 作者单位： | 河南省偃师市动物卫生监督所;上海启盛生物科技有限公司 |
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| 摘 要： | 从疑似犬瘟热(CD)病犬的脏器中分离到1株病毒,经间接免疫荧光试验、RT-PCR鉴定、红细胞凝集试验和对不同动物致病性试验,证实该病毒为犬瘟热病毒(CDV)强毒株,命名为CDV YD株。对H基因序列的测定和分析表明,YD株H基因与国内强毒株更接近,核苷酸同源性为98.4%~99.7%,氨基酸同源性为97.8%~99.7%,而与疫苗株核苷酸同源性较远为91.2%~91.5%,氨基酸同源性为90.3%~91.2%。推导的H蛋白氨基酸序列中有9个潜在的N连接糖基化位点(N-X-S/T),可能与病毒体外复制和中和抗体有关,另外有12个保守的半胱氨酸(Cys)残基,对H蛋白二级结构起重要作用;根据H基因核苷酸序列绘制的进化树表明,CDV YD株属于国内流行基因型:Asia-1型。该研究为了解当前中国CDV流行变异情况和犬瘟热疫苗的研发提供了数据。
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| 关 键 词： | 犬瘟热病毒 分离 鉴定 H基因 序列分析 |
Isolation and identification of caine distemper virus strain YD and sequence analysis of its H gene |
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| ZHANG Jianpu,MA Lili,ZHOU Lifeng.Isolation and identification of caine distemper virus strain YD and sequence analysis of its H gene[J].Animal Husbandry & Veterinary Medicine,2021(4):69-75. |
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| Authors: | ZHANG Jianpu MA Lili ZHOU Lifeng |
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| Institution: | (Yanshi Animal Health Supervision Institute,Yanshi 471900,China;Shanghai Qisheng Biological Technology Co.,Ltd.,Shanghai 201203,China) |
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| Abstract: | A strain of virus was isolated from the organs of a dog suspected with canine distemper(CD).The virulent virus,designated as YD,was identified as canine distemper virus(CDV)by indirect immunofluorescence assay,RT-PCR,haemagglutination and pathogenesis in different animals.Analysis of the H gene derived from the virulent strain revealed that the YD strain had 98.4%-99.7%nucleotide sequence homology and 97.8%-99.7% homology in putative amino acid sequences,especially sharing a high degree of homology with the Chinese virulent strain;but,compared with the existing vaccine strains,the homologies of YD strain were 91.2%-91.5% in the nucleotide sequence and 90.3%-91.2% in the amino acid sequence.Nine potential sites for N-linked glycosylation which might be related to virus neutralization by antibodies and replication in vitro were identified in the H protein of the YD strain.In addition,twelve conservative cysteine residues known to be important for the secondary structure of the H protein were also found in its predicted amino acid sequences.Phylogenetic analysis of the N and H genes showed that the YD strain belonged with the Asia-1 group,the predominant genotype in China.This study offered a basis for a better understanding of genetic variation of CDV in China and for development of CD vaccine. |
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| Keywords: | canine distemper virus isolation identification H gene sequence analysis |
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