| PRRSV感染仔猪肾组织的超微病理变化分析 |
| |
| 引用本文： | 罗传真,张羽晨,娄江城,刘晓丽,谷长勤,张万坡,程国富,胡薛英.PRRSV感染仔猪肾组织的超微病理变化分析[J].畜牧兽医学报,2021,52(4):1079-1085. |
| |
| 作者姓名： | 罗传真 张羽晨 娄江城 刘晓丽 谷长勤 张万坡 程国富 胡薛英 |
| |
| 作者单位： | 华中农业大学动物医学院, 武汉 430070 |
| |
| 基金项目： | 国家重点研发计划项目（2017YFD0501100） |
| |
| 摘 要： | 猪繁殖与呼吸综合征病毒（PRRSV）是能引起仔猪高死亡率的疾病，给养猪业带来极大的经济损失。本研究对PRRSV感染仔猪肾组织的病毒定位及产生的超微病理变化进行观察，以期了解PRRSV对肾组织细胞以及血液循环的影响。主要采用透射电镜技术结合血清生化检测、免疫组织化学染色（IHC），对仔猪感染PRRSV后肾组织进行超微病理学观察及分析。结果发现，PRRSV感染后引起肾组织损伤，病毒主要分布于肾小球、坏死的肾小管上皮细胞胞质及巨噬细胞胞质内；肾小球内足细胞足突广泛融合或微绒毛化，内皮细胞肿胀；肾小管上皮细胞线粒体肿胀、嵴断裂、溶解；间质炎性细胞浸润。PRRSV通过巨噬细胞内复制随血液扩散至肾组织，引起损伤；根据透射电镜观察结果，足细胞足突融合，肾小球血管内皮细胞损伤引起微血栓形成，说明PRRSV感染仔猪后影响了肾小球滤过率，同时，对肾血液微循环系统造成损伤。本研究为阐明PRRSV的感染致病机制提供理论基础。
|
| 关 键 词： | 猪繁殖与呼吸综合征病毒 肾 超微结构 电镜 |
| 收稿时间： | 2020-09-15 |
Ultrastructural Changes in Renal Tissue of Piglets Infected with PRRSV |
| |
| LUO Chuanzhen,ZHANG Yuchen,LOU Jiangcheng,LIU Xiaoli,GU Changqin,ZHANG Wanpo,CHENG Guofu,HU Xueying.Ultrastructural Changes in Renal Tissue of Piglets Infected with PRRSV[J].Acta Veterinaria et Zootechnica Sinica,2021,52(4):1079-1085. |
| |
| Authors: | LUO Chuanzhen ZHANG Yuchen LOU Jiangcheng LIU Xiaoli GU Changqin ZHANG Wanpo CHENG Guofu HU Xueying |
| |
| Institution: | College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China |
| |
| Abstract: | Porcine reproductive and respiratory syndrome virus (PRRSV) is a disease that causes high piglet mortality and brings great economic loss to the pig industry. In this study, the localization and ultrastructure changes of virus in piglets’ kidney infected with PRRSV were observed to understand the effect of PRRSV on renal tissue cells and blood circulation. Transmission electron microscopy (TEM) combined with serum biochemical test and immunohistochemistry (IHC) was used to observe and analyze the renal tissue of piglets infected with PRRSV. It was found that PRRSV infection caused renal tissue damage, and the virus was mainly distributed in the cytoplasm of glomeruli, necrotic renal tubular epithelial cells and macrophages. Extensive fusion of podocyte processes or formation of microvilli and swelling of endothelial cells were found. The mitochondria in the renal tubules epithelial cells were swollen, cristae fractured and dissolved; In interstitial, inflammatory cell infiltrated. According to the observation results, PRRSV caused serious damage to the kidney, podocyte process fusion resulted in increased glomerular permeability and the injury of glomerular vascular endothelial cells leaded to microthrombus formation. These results indicated that PRRSV affected the glomerular filtration rate and blood circulation system of piglets. It provided a theoretical basis for elucidating the pathogenic mechanism of PRRSV infection. |
| |
| Keywords: | porcine reproductive and respiratory syndrome virus(PRRSV) ultrastructure kidney electron microscopy (SEM) |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
| 点击此处可从《畜牧兽医学报》下载免费的PDF全文 |
|
