| 牛肺泡表面活性蛋白A的分离纯化及抗菌活性分析 |
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| 引用本文： | 李楠,张建楼,张永红,郑志强,霍珊珊,仲飞,翟向和.牛肺泡表面活性蛋白A的分离纯化及抗菌活性分析[J].畜牧兽医学报,2021,52(2):498-505. |
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| 作者姓名： | 李楠 张建楼 张永红 郑志强 霍珊珊 仲飞 翟向和 |
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| 作者单位： | 河北农业大学动物科技学院/动物医学院, 河北省兽医生物技术创新中心, 河北省牛羊胚胎工程技术创新中心, 保定 071001 |
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| 基金项目： | 河北省自然科学基金(C2013204130);家畜疫病病原生物学国家重点实验室开放课题(SKLVEB2013KFKT022);河北省高等学校科学技术研究项目(ZD20131056)。 |
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| 摘 要： | 旨在分离纯化牛天然肺泡表面活性蛋白A(SP-A)和分析其抗菌活性,首先通过共价交联法制备出可特异吸附SP-A的麦芽糖-Sepharose(MS)胶粒,然后用MS胶粒从牛肺泡洗出液中吸附牛SP-A,从而得到牛SP-A.SDS-PAGE和Western blot检测表明,牛SP-A单体的相对分子质量为30 ku,二聚体为6...
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| 关 键 词： | 牛表面活性蛋白A 分离纯化 麦芽糖-Sepharose胶粒 抗菌活性 |
| 收稿时间： | 2020-07-31 |
Preparation of Bovine Surfactant Protein A and Its Antibacterial Activities |
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| LI Nan,ZHANG Jianlou,ZHANG Yonghong,ZHENG Zhiqiang,HUO Shanshan,ZHONG Fei,ZHAI Xianghe.Preparation of Bovine Surfactant Protein A and Its Antibacterial Activities[J].Acta Veterinaria et Zootechnica Sinica,2021,52(2):498-505. |
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| Authors: | LI Nan ZHANG Jianlou ZHANG Yonghong ZHENG Zhiqiang HUO Shanshan ZHONG Fei ZHAI Xianghe |
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| Institution: | College of Animal Science and Technology/College of Veterinary Medicine, Agricultural University of Hebei, Hebei Veterinary Biotechnology Innovation Center, Hebei Technology Innovation Center of Cattle and Sheep Embryo, Baoding 071001, China |
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| Abstract: | To isolate natural bovine lung surfactant protein A(SP-A)and analyze its antibacterial activities,the maltose-sepharose(MS)beads specifically binding bovine SP-A was prepared by the covalently crosslinking method.The MS beads were used to absorb the bovine SP-A from bovine lung lavage.SDS-PAGE and Western blot detections showed that two forms of SP-A,30 ku monomer and 60 ku dimer,were detected in the bovine SP-A preparation.The SP-A was identified as possessing the ability to aggregate E.coli,suggesting it possesses biological activity.The antibacterial experiment showed that SP-A significantly inhibited the growth of Staphylococcus aureus,Streptococcus agalactiae and the pathogenic E.coli,especially for Gram-negative bacteria with high inhibition activity,indicating that the bovine SP-A prepared in this study possesses antibacterial activities.This study provided necessary conditions for further investigation on SP-A biological characteristics and antibacterial activities,as well as opened up a new way for the development of SP-A antibacterial reagent. |
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| Keywords: | bovine surfactant protein A isolation and purification meltose-sepharose beads antibacterial activity |
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