| 10种中药成分对单层鸡胚成纤维细胞增殖的影响 |
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| 引用本文： | 孔祥峰,胡元亮,刘家国,张宝康,陈玉库,王德云.10种中药成分对单层鸡胚成纤维细胞增殖的影响[J].南京农业大学学报,2003,26(2):84-87. |
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| 作者姓名： | 孔祥峰 胡元亮 刘家国 张宝康 陈玉库 王德云 |
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| 作者单位： | 南京农业大学中兽医学研究室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 0 70 5 66) |
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| 摘 要： | 将安全浓度范围内的黄芪多糖、当归多糖、蜂胶多糖、淫羊藿多糖、板蓝根多糖、黄芪黄酮、蜂胶黄酮、淫羊藿黄酮、黄芪皂苷和人参皂苷等 10种中药成分分别加入已培养 2 4h、刚形成单层的鸡胚成纤维细胞 (CEF)中 ,在加药后12 ,2 4 ,36 ,4 8和 6 0h用MTT法测定它们对CEF增殖的影响。结果表明 ,黄芪皂苷、黄芪多糖、板蓝根多糖和蜂胶黄酮主要表现为促进增殖 ;淫羊藿多糖主要具抑制效应 ;其他 5种中药成分在某些浓度和时间点能促进增殖 ,而在某些浓度和时间点则抑制增殖 ,并有一定的量效和时效关系。
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| 关 键 词： | 中药成分 鸡胚成纤维细胞 细胞增殖 |
| 文章编号： | 1000-2030(2003)02-0084-04 |
| 修稿时间： | 2002年9月18日 |
Effects of ten Chinese herbal medicinal ingredients on proliferation of monolayer chick embryo fibroblast |
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| KONG Xiang feng,HU Yuan liang ,LIU Jia guo,ZHANG Bao kang,CHEN Yu ku,WANG De yun.Effects of ten Chinese herbal medicinal ingredients on proliferation of monolayer chick embryo fibroblast[J].Journal of Nanjing Agricultural University,2003,26(2):84-87. |
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| Authors: | KONG Xiang feng HU Yuan liang LIU Jia guo ZHANG Bao kang CHEN Yu ku WANG De yun |
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| Institution: | KONG Xiang feng,HU Yuan liang *,LIU Jia guo,ZHANG Bao kang,CHEN Yu ku,WANG De yun |
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| Abstract: | Ten Chinese herbal medicinal ingredients,astragalus polysaccharide(APS),Chinese angelica polysaccharide(CAPS),propolis polysaccharide(PPS),epimedium polysaccharide(EPS),isatis root polysaccharide(IRPS),astragalus flavone(AF),propolis flavone(PF),epimedium flavone(EF),astragalosides(AS)and ginsenosides(GS),within safety concentration were respectively added onto monolayer of chick embryo fibroblast(CEF)which had been cultured for 24 hours.12,24,36,48 and 60 hours after administration their effects on CEF proliferation were assayed by MTT methods.The results showed that AS,APS,IRPS and PF mainly promoted and EPS inhibited the cell proliferating.The other five stimulated or restrained the proliferation at some concentrations and time points,which had relations to dose and time effect. |
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| Keywords: | Chinese herbal medicinal ingredient chick embryo fibroblast cell proliferation |
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