| 河南省地黄病毒病初步鉴定 |
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| 引用本文： | 张振臣,张丽芳,乔奇,王永江,靳秀兰.河南省地黄病毒病初步鉴定[J].植物病理学报,2004,34(5):395-399. |
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| 作者姓名： | 张振臣 张丽芳 乔奇 王永江 靳秀兰 |
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| 作者单位： | 河南省农业科学院植物保护研究所, 郑州 450002 |
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| 基金项目： | 河南省高校杰出科研创新人才工程项目，国家科技攻关项目 |
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| 摘 要： | 利用血清学、RT-PCR并结合核苷酸序列测定等方法,对河南省地黄病毒病进行了初步鉴定。结果表明,烟草花叶病毒(TMV)为侵染地黄的主要病毒;对TMV地黄分离物(TMV-RH) CP基因的序列分析结果表明,TMV-RH与TMV-U1株系CP基因的核苷酸同源性为86.5%,氨基酸同源性为94.3%;与已发表的TMV其它株系CP基因的核苷酸同源性在76.3%~88.5%之间,氨基酸同源性在79.3%~95.0%之间,同源性较低。根据不同株系CP的氨基酸序列进化树分析,推测该分离物可能为TMV的一个新株系。
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| 关 键 词： | 地黄 病毒病 烟草花叶病毒 株系 |
| 文章编号： | 0412-0914(2004)05-0395-05 |
| 修稿时间： | 2003年12月8日 |
Identification of viral pathogens of Rehmannia glutinosa disease in Henan Province |
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| ZHANG Zhen chen,ZHANG Li fang,QIAO Qi,WANG Yong jiang,JIN Xiu lan.Identification of viral pathogens of Rehmannia glutinosa disease in Henan Province[J].Acta Phytopathologica Sinica,2004,34(5):395-399. |
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| Authors: | ZHANG Zhen chen ZHANG Li fang QIAO Qi WANG Yong jiang JIN Xiu lan |
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| Institution: | Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China |
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| Abstract: | Virus species infected Rehmannia glutinosa were detected by using ELISA, RT PCR and nucleotide (nt) sequence analysis of coat protein (CP) gene. The results revealed that Tobacco mosaic virus (TMV) was the major virus infecting Rehmannia glutinosa in Henan Province. CP gene of TMV from Rehmannia glutinosa (TMV RH) was cloned and sequenced. The sequence analysis showed that the nt and amino acid (aa) sequence of TMV RH CP were 86.5% and 94.3% identical to TMV U1 strain, and were 76.3% to 88.5% and 79.3% to 95.0% identical to other TMV strains, respectively. According to the results of phylogenetic tree generated based on aa sequences of CP genes, it is supposed that TMV RH may be a new strain of TMV. |
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| Keywords: | Rehmannia glutinosa virus disease Tobacco mosaic virus strain |
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