| 利用基于重复序列PCR的标记和A-PAGE鉴定小麦背景中的黑麦染色体片段 |
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| 引用本文： | 何道文,彭正松,伍碧华.利用基于重复序列PCR的标记和A-PAGE鉴定小麦背景中的黑麦染色体片段[J].西南农业大学学报,2007,29(5):66-69. |
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| 作者姓名： | 何道文 彭正松 伍碧华 |
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| 作者单位： | [1]西华师范大学生命科学学院,四川南充637002 [2]四川农业大学小麦研究所,四川雅安625014 |
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| 基金项目： | 国家自然科学基金资助项目（30571139）;四川省科技厅基础应用项目（04JY029-055-1）. |
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| 摘 要： | 利用基于重复序列PCR的标记和酸性丙烯酰胺凝胶电泳（A-PAGE）对小麦品种中国春与秦岭黑麦杂交后代（BC2F4）共75份材料进行了筛选，鉴定含有黑麦成分的株系。根据黑麦特异重复序列pSc20H设计特异引物，用PCR方法从75个株系中筛选出30份含有黑麦成分的材料。从这30份材料中，用A-PAGE的方法鉴定出10个株系为1RS／1BL易住系。实验结果表明，用基于PCR的标记能快速准确地对小麦背景中外源染色质的鉴定。结合其它方法还能进行小片段移位的鉴定。
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| 关 键 词： | 小麦 黑麦染色体片段 重复序列PCR 酸性丙烯酰胺凝胶电泳 |
| 文章编号： | 1000-2642（2007）05-0066-04 |
| 修稿时间： | 2007-04-25 |
Use of rep-PCR-Based Markers and A-PAGE to Identify Rye Chromosomal Segments in Wheat Background |
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| HE Dao-wen^,PENG Zheng-song^,WU Bi-hua^.Use of rep-PCR-Based Markers and A-PAGE to Identify Rye Chromosomal Segments in Wheat Background[J].Journal of Southwest Agricultural University,2007,29(5):66-69. |
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| Authors: | HE Dao-wen^ PENG Zheng-song^ WU Bi-hua^ |
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| Institution: | 1.School of Life Science, China West Normal University, Nanchong Sichuan 637002, China ; 2. TriticeaeResearchlnstitute , SichuanAgriculturalUniversity,Yaan Sichuan 625014, China |
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| Abstract: | PCR-based markers and A-PAGE were used to identify rye chromosomal segments in the advanced wheat lines (BC2F4), resulting from a cross between common wheat cultivar Chinese Spring and Qinlin rye. Of the 75 wheat lines investigated, 30 were found to contain chromosomal segments of rye using a PCR-based marker which was designed according to sequence of pSc20H. Of the 30 wheat lines, 10 were confirmed to be 1RS/1BL translocations using A-PAGE analysis. These results in the study indicate that alien chromatin in wheat can be detected rapidlyt and accurately using PCR-based markers. Combined with other methods, PCR-based markers can be used to investigate small alien segments introduced into wheat. |
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| Keywords: | wheat rye chromosomal segments rep-PCR A-PAGE |
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