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| 6株猪流行性腹泻病毒贵州株S基因序列分析 | |
| 引用本文： | 毛黎红,张双翔,周碧君,王伟,胡兴义,程振涛,文明,王开功.6株猪流行性腹泻病毒贵州株S基因序列分析[J].中国畜牧兽医,2017,44(6):1621-1629. |
| 作者姓名： | 毛黎红 张双翔 周碧君 王伟 胡兴义 程振涛 文明 王开功 |
| 作者单位： | 1. 贵州省贵阳市花溪区农业局, 贵阳 550001; 2. 贵州省动物疫病预防控制中心, 贵阳 550001; 3. 贵州大学动物科学学院, 贵阳 550025 |
| 基金项目： | 贵州省科学技术厅农业攻关项目"贵州省仔猪腹泻性疫病风险分析及综合防控技术研究"（黔科合NY[2015]3009-1号）；贵州省生猪质量安全工程技术研究中心建设项目"生猪健康养殖实验室建设与技术研发"黔科合农C字[2011]4022号；贵州省农业委员会科技计划项"2015年贵州省重大动物疫病省级定点监测"[2015-11号] |
| 摘 要： | 为了解贵州省猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）S基因的特点，掌握其遗传变异情况，本试验设计3对特异性引物对6株PEDV贵州株S基因进行全基因RT-PCR扩增、克隆及序列测定；应用生物信息学软件将6株PEDV贵州流行株与参考毒株进行同源性与系统进化分析。结果显示，6株PEDV贵州流行株S基因的全基因长为4 161 bp，编码1 387个氨基酸，与国内外PEDV参考毒株核苷酸同源性在93.3%~98.8%之间，氨基酸同源性在91.6%~98.9%之间；进化树分析结果显示，6株流行毒株与美国毒株、越南毒株和山东毒株亲缘关系较近；与CV777株、LZC、Brl、83P-5亲缘关系较远。结果表明，PEDV贵州地方流行毒株S基因编码的氨基酸存在一定程度变化，近年来呈现变异趋势。本研究可为贵州省PEDV的防控提供一定的理论依据。 |
| 关 键 词： | 猪流行性腹泻 S基因 生物信息学分析 |
| 收稿时间： | 2016-10-26 |
Sequence Analysis of S Gene of 6 Strains of Porcine Epidemic Diarrhea Virus from Guizhou |
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| MAO Li-hong,ZHANG Shuang-xiang,ZHOU Bi-jun,WANG Wei,HU Xing-yi,CHENG Zheng-tao,WEN Ming,WANG Kai-gong.Sequence Analysis of S Gene of 6 Strains of Porcine Epidemic Diarrhea Virus from Guizhou[J].China Animal Husbandry & Veterinary Medicine,2017,44(6):1621-1629. | |
| Authors: | MAO Li-hong ZHANG Shuang-xiang ZHOU Bi-jun WANG Wei HU Xing-yi CHENG Zheng-tao WEN Ming WANG Kai-gong |
| Institution: | 1. Huaxi District Agricultural Bureau of Guizhou, Guiyang 550001, China; 2. Animal Disease Control and Prevention Center of Guizhou, Guiyang 550001, China; 3. College of Animal Science, Guizhou University, Guiyang 550025, China |
| Abstract: | In order to understand the characteristics of S gene of porcine epidemic diarrhea virus (PEDV) in Guizhou province, and grasp the genetic variation of PEDV, according to 3 pairs of specific primers designed in the test, S gene of 6 strains of PEDV from Guizhou were amplified by RT-PCR, cloned and sequenced. Nucleotide sequence and phylogenetic tree of 6 strains of PEDV from Guizhou and reference strains were analyzed by biological information software. The result showed that the length of S gene genome of 6 strains of PEDV from Guizhou was 4 161 bp, encoding 1 387 amino acids, and the nucleotide homologies of domestic and foreign PEDV reference were from 93.3% to 98.8%, the amino acid identity were from 91.6% to 98.9%. Phylogenetic tree analysis results showed that the 6 strains were close to the American strain, Vietnam strain and the Shandong strain.They were far from the CV777, LZC, Brl and 83P-5.The experiment showed that the S gene of PEDV in Guizhou had a certain degree of amino acid change in recent year with a trend of variation. The study provided a theoretical basis for the prevention and control of PEDV in Guizhou province. |
| Keywords: | porcine epidemic diarrhea S gene bioinformatics analysis |
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