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| 利用miRNA芯片筛选不同月龄香猪肝脏中差异表达的miRNA | |
| 引用本文： | 李升,许亚楠,牛熙,冉雪琴.利用miRNA芯片筛选不同月龄香猪肝脏中差异表达的miRNA[J].中国畜牧兽医,2017,44(3):673-678. |
| 作者姓名： | 李升 许亚楠 牛熙 冉雪琴 |
| 作者单位： | 1. 贵州大学农业生物工程研究院, 贵阳 550025; 2. 贵州大学动物科学学院, 贵阳 550025 |
| 基金项目： | 国家自然科学基金（31401091）；贵州省科学技术基金（黔科合J字[2013]2100号） |
| 摘 要： | 为研究miRNA在香猪发育时期的调控功能，试验对3和7月龄香猪肝脏组织中存在表达差异的miRNA进行了筛选。提取3和7月龄香猪肝脏组织总RNA，通过与miRNA芯片杂交，经统计学分析，以变化倍数≥2倍（P<0.05）为阈值，筛选3和7月龄香猪肝脏中表达丰度差异的miRNA，并运用实时荧光定量PCR进行验证。结果发现，与3月龄相比，7月龄香猪肝脏中有11条miRNA表达下调和9条miRNA表达上调，下调倍数最多的是miR-27b，上调倍数最多的是miR-155。对选取的4条差异表达的miRNA进行实时荧光定量PCR反应，结果显示，4条miRNA表达变化趋势与芯片结果一致。本研究结果表明，不同月龄香猪肝脏中的miRNA表达丰度有差异，其中一些可能参与了机体生长发育时期肝细胞的脂肪代谢过程。 |
| 关 键 词： | miRNA表达谱 香猪 肝脏 |
| 收稿时间： | 2016-09-07 |
Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray |
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| LI Sheng,XU Ya-nan,NIU Xi,RAN Xue-qin.Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray[J].China Animal Husbandry & Veterinary Medicine,2017,44(3):673-678. | |
| Authors: | LI Sheng XU Ya-nan NIU Xi RAN Xue-qin |
| Institution: | 1. Institute of Agro-bio Engineering, Guizhou University, Guiyang 550025, China; 2. Faculty of Animal Science, Guizhou University, Guiyang 550025, China |
| Abstract: | To determine the roles of miRNA in the developmental stages of Xiang pig,we investigated the miRNA expression patterns in different months old of Xiang pigs.The total RNA from 3 and 7 months old of Xiang pigs were collected separately. Then,the total RNA were hybridized with the miRNA microarray and the threshold (fold-change>2, P<0.05) were used to identify the different expression miRNA. The quantitative Real-time PCR was performed to verify the microarray result. Compared to the 3 months old group,there were 11 miRNA down regulated and 9 miRNA were up regulated in the 7 months old group. The lowest down regulated miRNA was miR-27b whereas the highest up regulated one was miR-155.The result of quantitative Real-time PCR consistented with the microarray result. The result suggested that some of the dysregulated expression of miRNA might participate in the lipid metabolism in hepatic cells. |
| Keywords: | miRNA expression profile Xiang pig liver |
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