| 牛GUCY1A3和SFXN1基因的克隆及生物信息学分析 |
| |
| 引用本文： | 靳聪飞,梁婷玉,刘新峰,郭宏.牛GUCY1A3和SFXN1基因的克隆及生物信息学分析[J].中国畜牧兽医,2017,44(2):357-364. |
| |
| 作者姓名： | 靳聪飞 梁婷玉 刘新峰 郭宏 |
| |
| 作者单位： | 天津农学院动物科学与动物医学学院, 天津 300384 |
| |
| 基金项目： | 高产转基因肉牛新品种培育（2014ZX08007-002） |
| |
| 摘 要： | 试验以人GUCY1A3和SFXN1基因序列为探针，BLAST获得同源性较高的牛的表达序列标签（ESTs）序列及部分mRNA序列。通过RT-PCR方法从牛肌肉及脑组织克隆cDNA序列与牛表达序列标签进行拼接，获得牛GUCY1A3和SFXN1基因，并对其进行了生物学特性分析。序列分析表明，牛GUCY1A3基因编码区长2 076 bp，编码691个氨基酸；牛SFXN1基因编码区长969 bp，编码322个氨基酸。氨基酸序列分析表明，GUCY1A3蛋白磷酸化位点分布于丝氨酸（Ser）、酪氨酸（Tyr）和苏氨酸（Thr）残基上，主要分布在细胞质中，不属于分泌蛋白，二级结构预测以α螺旋为主，保守结构域含1个CYCc功能结构域；SFXN1蛋白磷酸化位点分布于丝氨酸（Ser）、酪氨酸（Tyr）和苏氨酸（Thr）残基上，主要分布在内质网和浆膜上，属于跨膜蛋白，二级结构以α螺旋为主，保守结构域含1段低复杂度序列和3段跨膜螺旋区。试验结果为进一步研究牛GUCY1A3和SFXN1基因的表达调控机制与功能奠定了基础。
|
| 关 键 词： | 牛 GUCY1A3基因 SFXN1基因 电子克隆 生物信息学分析 |
| 收稿时间： | 2016-07-18 |
Cloning and Bioinformatics Analysis of GUCY1A3 and SFXN1 Genes in Cattle |
| |
| JIN Cong-fei,LIANG Ting-yu,LIU Xin-feng,GUO Hong.Cloning and Bioinformatics Analysis of GUCY1A3 and SFXN1 Genes in Cattle[J].China Animal Husbandry & Veterinary Medicine,2017,44(2):357-364. |
| |
| Authors: | JIN Cong-fei LIANG Ting-yu LIU Xin-feng GUO Hong |
| |
| Institution: | College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China |
| |
| Abstract: | In this study, the human GUCY1A3 and SFXN1 genes sequences in GenBank were chosen as probes to BLAST and got mRNA,and expressed sequence tags (ESTs) of bovine GUCY1A3 and SFXN1 genes. The bovine GUCY1A3 and SFXN1 genes were amplified by sequencing assembling of ESTs and cDNA sequences using RT-PCR. Then,we analyzed encoding proteins of bovine GUCY1A3 and SFXN1 genes with bioinformatics methods. Sequence analysis showed that the bovine GUCY1A3 gene CDS sequence was 2 076 bp,which encoded 691 amino acids, and the bovine SFXN1 gene contained a CDS region of 969 bp, which encoded 322 amino acids. The GUCY1A3 protein contained a CYCc domain, and the phosphorylation sites located in threonine, serine and tyrosine residue. The subcellular localization of GUCY1A3 protein was in the cytoplasm and it did not belong to the secreted protein. The secondary structure of GUCY1A3 protein was mainly composed of alpha helix. The SFXN1 protein contained three transmembrane helical regions and one low complexity sequence, and the phosphorylation sites located in serine, tyrosine and threonine residue. The subcellular localization of SFXN1 protein was in the endoplasmic reticulum and membrane, and it belonged to the transmembrane protein. The secondary structure of SFXN1 was mainly composed of alpha helix. The results laid the foundation for further studies of expression regulation mechanism and function of GUCY1A3 and SFXN1 genes in cattle. |
| |
| Keywords: | cattle GUCY1A3 gene SFXN1 gene cloning bioinformatics analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
